Author | Batista, Cassiano Martin | |
Author | Saad, Felipe | |
Author | Ceccoti, Stephane Pini Costa | |
Author | Eger, Iriane | |
Author | Soares, Maurilio José | |
Access date | 2018-09-20T15:36:58Z | |
Available date | 2018-09-20T15:36:58Z | |
Document date | 2018 | |
Citation | BATISTA, Cassiano Martin et al. Subcellular localisation of FLAG tagged enzymes of the dynamic protein S-palmitoylation cycle of Trypanosoma cruzi epimastigotes. Mem. Inst. Oswaldo Cruz, Rio de Janeiro, v.113, n.8, 2018. | pt_BR |
ISSN | 0074-0276 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/28942 | |
Language | por | pt_BR |
Publisher | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Trypanosoma cruzi | pt_BR |
Subject in Portuguese | Trypanosomatina | pt_BR |
Title | Subcellular localisation of FLAG tagged enzymes of the dynamic protein S-palmitoylation cycle of Trypanosoma cruzi epimastigotes | pt_BR |
Type | Article | pt_BR |
DOI | 10.1590/0074-02760180086 | |
Abstract | Dynamic S-palmitoylation of proteins is the addition of palmitic acid by zDHHC palmitoyl transferases (PATs) and depalmitoylation by palmitoyl protein thioesterases (PPTs). A putative PAT (TcPAT1) has been previously identified in Trypanosoma cruzi, the etiological agent of Chagas disease. Here we analyse other 14 putative TcPATs and 2 PPTs in the parasite genome. T. cruzi cell lines expressing TcPATs and TcPPTs plus a FLAG tag at the C terminus were produced for most enzymes, with positive detection by indirect immunofluorescence. Overexpressed TcPATs were mostly found as single spots at the parasite anterior end, while the TcPPTs were dispersed throughout the parasite body. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Celular. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Celular. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Celular. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Universidade Estadual de Ponta Grossa. Departamento de Biologia Geral. Ponta Grossa, PR, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Celular. Curitiba, PR, Brasil. | pt_BR |
Subject | Lipoylation | pt_BR |
Subject | Palmitic Acid | pt_BR |
Subject | Gene Expression | pt_BR |
Subject in Spanish | Lipoilación | pt_BR |
Subject in Spanish | Ácido Palmítico | pt_BR |
Subject in Spanish | Expresión Génica | pt_BR |
DeCS | Lipoilação | pt_BR |
DeCS | Ácido Palmítico | pt_BR |
DeCS | Expressão Gênica | pt_BR |
e-ISSN | 1678-8060 | |