| Author | Silva, P. L. | |
| Author | Garcia, C. S. N. B. | |
| Author | Maronas, P. A. | |
| Author | Cagido, V. R. | |
| Author | Negri, E. M. | |
| Author | Damaceno-Rodrigues, N. R. | |
| Author | Ventura, G. M. | |
| Author | Bozza, Patrícia T. | |
| Author | Zin, W. A. | |
| Author | Capelozzi, V. L. | |
| Author | Pelosi, P. | |
| Author | Rocco, P. R. M. | |
| Access date | 2018-09-23T15:15:05Z | |
| Available date | 2018-09-23T15:15:05Z | |
| Document date | 2009 | |
| Citation | SILVA, P. L. et al. Early short-term versus prolonged low-dose methylprednisolone therapy in acute lung injury. Eur. Respir. J., v.33, p.634-645, 2009. | pt_BR |
| ISSN | 0903-1936 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/28999 | |
| Language | eng | pt_BR |
| Publisher | European Respiratory Society | pt_BR |
| Rights | restricted access | |
| Subject in Portuguese | lesão pulmonar aguda | pt_BR |
| Subject in Portuguese | colágeno | pt_BR |
| Subject in Portuguese | Transcortina | pt_BR |
| Subject in Portuguese | elastância | pt_BR |
| Subject in Portuguese | histologia pulmonar | pt_BR |
| Title | Early short-term versus prolonged low-dose methylprednisolone therapy in acute lung injury | pt_BR |
| Type | Article | |
| DOI | 10.1183/09031936.00052408 | |
| Abstract | The present study compared the effects of early short-term with prolonged low-dose corticosteroid therapy in acute lung injury (ALI). In total, 120 BALB/c mice were randomly divided into five groups. In the control group, saline was intratracheally (i.t.) instilled. In the ALI group, mice received Escherichia coli lipopolysaccharide (10 microg i.t.). ALI animals were further randomised into four subgroups to receive saline (0.1 mL i.v.) or methylprednisolone (2 mg x kg(-1) i.v.) at 6 h, 24 h or daily (for 7 days, beginning at day 1). At 1, 3 and 8 weeks, in vivo and in vitro lung mechanics and histology (light and electron microscopy), collagen and elastic fibre content, cytokines in bronchoalveolar lavage fluid and the expression of matrix metalloproteinase (MMP)-9 and -2 were measured. In vivo (static elastance and viscoelastic pressure) and in vitro (tissue elastance and resistance) lung mechanics, alveolar collapse, cell infiltration, collagen and elastic fibre content and the expression of MMP-9 and MMP-2 were increased in ALI at 1 week. Methylprednisolone led to a complete resolution of lung mechanics, avoided fibroelastogenesis and the increase in the expression of MMP-9 and MMP-2 independent of steroid treatment design. Thus, early short-term, low-dose methylprednisolone is as effective as prolonged therapy in acute lung injury. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Investigação Pulmonar. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Investigação Pulmonar. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Investigação Pulmonar. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Fisiologia Pulmonar. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade de São Paulo. Faculdade de Medicina. Departamento de Patologia. São Paulo, SP, Brasil. | pt_BR |
| Affilliation | Universidade de São Paulo. Faculdade de Medicina. Departamento de Patologia. São Paulo, SP, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade de São Paulo. Faculdade de Medicina. Departamento de Patologia. São Paulo, SP, Brasil. | pt_BR |
| Affilliation | University of Insubria. Dept. of Ambient, Health and Safety. Varese, Italy. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Investigação Pulmonar. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Acute lung injury | pt_BR |
| Subject | collagen | pt_BR |
| Subject | corticosteroid | pt_BR |
| Subject | elastance | pt_BR |
| Subject | lung histology | pt_BR |
| Subject | matrix metalloproteinase | pt_BR |
| e-ISSN | 1399-3003 | |
| Embargo date | 2030-01-01 | |
