| Author | Cunha, Nathalie C. | |
| Author | Fonseca, Adivaldo H. | |
| Author | Rezende, Jania | |
| Author | Rozenthal, Tatiana | |
| Author | Favacho, Alexsandra R. M. | |
| Author | Barreira, Jairo D. | |
| Author | Massard, Carlos L. | |
| Author | Lemos, Elba R. S. | |
| Access date | 2018-09-25T11:16:41Z | |
| Available date | 2018-09-25T11:16:41Z | |
| Document date | 2009 | |
| Citation | CUNHA, Nathalie C. et al. First identification of natural infection of Rickettsia rickettsii in the Rhipicephalus sanguineus tick, in the State of Rio de Janeiro. Pesq. Vet. Bras., v.29, n.2, p.105-108, fev. 2009. | pt_BR |
| ISSN | 0100-736X | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/29030 | |
| Language | eng | pt_BR |
| Publisher | Colégio Brasileiro de Patologia Animal | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Rhipicephalus sanguineus | pt_BR |
| Subject in Portuguese | Acari | pt_BR |
| Subject in Portuguese | Ixodidae | pt_BR |
| Subject in Portuguese | Rickettsia rickettsii | pt_BR |
| Subject in Portuguese | análise molecular | pt_BR |
| Subject in Portuguese | febre maculosa brasileira | pt_BR |
| Title | First identification of natural infection of Rickettsia rickettsii in the Rhipicephalus sanguineus tick, in the State of Rio de Janeiro | pt_BR |
| Type | Article | |
| Abstract | The Brazilian Spotted Fever (BSF) is a zoonotic disease caused by Rickettsia rickettsii
and transmitted by ticks of the genus Amblyomma, more frequently, Amblyomma cajennense.
The aim of this paper was to report the first molecular detection of R. rickettsii on
R. sanguineus naturally infected in Rio de Janeiro, Brazil. Ticks were collected from
dogs in a rural region of Resende municipality, Rio de Janeiro State, Brazil (22o30’9.46'’S,
44o42’44.29'’WO), where occurred five human cases of BSF in 2006. The ticks were
identified under a stereoscopic microscope and separated in pools by stages, species
and sex. DNA extraction was carried out using QIAamp DNA Mini Kit (QIAGEN®). The
DNA was submitted to PCR amplification using 04 set of primers: Rr190.70p/Rr190.602n
(OmpA, 532bp), BG1-21/BG2-20 (OmpB, 650bp), Tz15/Tz16 (17 kDa protein-encoding
gene, 246bp) and RpCS.877p/RpCS.1258n (gltA, 381bp). PCR products were separated
by electrophoresis on 1% agarose gels and visualized under ultraviolet light with ethidium
bromide. PCR products of the expected sizes were purified by QIAquick® and sequenced
by ABI PRISM®. The generated nucleotide sequences were edited with using Bioedit®
software and compared with the corresponding homologous sequences available
through GenBank, using Discontiguous Mega Blast (http://www.ncbi.nlm.nih.gov). It
was confirmed R. rickettsii by sequencing of the material (GenBank FJ356230). The
molecular characterization of R. rickettsii in the tick R. sanguineus emphasizes the
role of dogs as carriers of ticks from the environment to home. Moreover, this result
suggests that there is a considerable chance for active participation of R. sanguineus
as one of tick species in the transmission of R. ricketsii to human being in the Brazilian
territory. | pt_BR |
| Affilliation | Universidade Federal Rural do Rio de Janeiro. Curso de Pós-Graduação em Ciências Veterinárias. Seropédica, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal Rural do Rio de Janeiro. Instituto de Veterinária. Departamento de Epidemiologia e Saúde Pública. Seropédica, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal Rural do Rio de Janeiro. Curso de Pós-Graduação em Ciências Veterinárias. Seropédica, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hantaviroses e Rickettsioses. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hantaviroses e Rickettsioses. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hantaviroses e Rickettsioses. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Universidade Federal Rural do Rio de Janeiro. Instituto de Veterinária. Departamento de Parasitologia Animal. Seropédica, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hantaviroses e Rickettsioses. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Subject | Rhipicephalus sanguineus | pt_BR |
| Subject | Acari | pt_BR |
| Subject | Ixodidae | pt_BR |
| Subject | Rickettsia rickettsii | pt_BR |
| Subject | molecular analysis | pt_BR |
| Subject | Brazilian spotted fever | pt_BR |
| e-ISSN | 1678-5150 | |
