| Author | Martins, Angelica N. | |
| Author | Medeiros, Sheila O. | |
| Author | Simonetti, Jose P. | |
| Author | Schatzmayr, Hermann G. | |
| Author | Tanuri, Amílcar | |
| Author | Brindeiro, Rodrigo M. | |
| Access date | 2018-09-25T12:20:01Z | |
| Available date | 2018-09-25T12:20:01Z | |
| Document date | 2008 | |
| Citation | MARTINS, Angelica N. et al. Phylogenetic and Genetic Analysis of Feline Immunodeficiency Virus gag, pol, and env Genes from Domestic Cats Undergoing Nucleoside Reverse Transcriptase Inhibitor Treatment or Treatment-Naïve Cats in Rio de Janeiro, Brazil. Journal of Virology, v.82, n.16, p.7863-7874, Aug. 2008. | pt_BR |
| ISSN | 0022-538X | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/29034 | |
| Language | eng | pt_BR |
| Publisher | American Society for Microbiology | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Análise Filogenética e Genética | pt_BR |
| Subject in Portuguese | Vírus da Imunodeficiência Felina | pt_BR |
| Subject in Portuguese | Gatos Domésticos | pt_BR |
| Subject in Portuguese | Nucleosídeos Tratamento Inibidor da Transcriptase Reversa | pt_BR |
| Subject in Portuguese | Treatment-Naïve | pt_BR |
| Subject in Portuguese | Rio de Janeiro | pt_BR |
| Subject in Portuguese | Brasil | pt_BR |
| Title | Phylogenetic and genetic analysis of feline immunodeficiency virus gag, pol, and env genes from domestic cats undergoing nucleoside reverse transcriptase inhibitor treatment or treatment-naïve cats in Rio de Janeiro, Brazil | pt_BR |
| Type | Article | |
| DOI | 10.1128/JVI.00310-08 | |
| Abstract | Feline immunodeficiency virus (FIV) is the Lentivirus responsible for an immunodeficiency-like disease in domestic cats (Felis catus). FIV is divided into five phylogenetic subtypes (A, B, C, D, and E), based on genetic diversity. Knowledge of the geographical distribution of subtypes is relevant for understanding different disease progressions and for vaccine development. In this study, viral sequences of 26 infected cats from Rio de Janeiro, 8 undergoing treatment with zidovudine (AZT) for at least 5 years, were successfully amplified from blood specimens. gag capsid (CA), pol reverse transcriptase (RT), and env gp120 (V3-V4) regions were analyzed to determine subtypes and to evaluate potential mutations related to antiretroviral drug resistance among treated cats. Subtyping based on phylogenetic analysis was performed by the neighbor-joining and maximum likelihood methods. All of the sequences clustered with subtype B in the three regions, exhibiting low genetic variability. Additionally, we found evidence that the same virus is circulating in animals in close contact. The analysis of FIV RT sequences identified two new putative mutations related to drug resistance located in the RT "finger" domain, which has 60% identity to human immunodeficiency virus (HIV) sequence. Amino acid change K-->R at codons 64 and 69 was found in 25% and 37.5% of the treated animals, respectively. These signatures were comparable to K65R and K70R thymidine-associated mutations found in the HIV-1 HXB2 counterpart. This finding strongly suggests a position correlation between the mutations found in FIV and the K65R and K70R substitutions from drug-resistant HIV-1 strains. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Departamento de Genética. Laboratório de Virologia Molecular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Departamento de Genética. Laboratório de Virologia Molecular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Laboratório de Flavivírus. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Departamento de Genética. Laboratório de Virologia Molecular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Departamento de Genética. Laboratório de Virologia Molecular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Feline Immunodeficiency Virus | pt_BR |
| Subject | domestic cats | pt_BR |
| Subject | Phylogenetic and Genetic Analysis | pt_BR |
| Subject | Reverse Transcriptase Inhibitor Treatment | pt_BR |
| Subject | Treatment-Naïve | pt_BR |
| Subject | Rio de Janeiro | pt_BR |
| Subject | Brazil | pt_BR |
| e-ISSN | 1098-5514 | |
