| Author | Fontes, Amanda Nogueira Brum | |
| Author | Sakamuri, Rama Murty | |
| Author | Baptista, Ida Maria Foschiani Dias | |
| Author | Ura, Somei | |
| Author | Moraes, Milton Ozório | |
| Author | Martinez, Alejandra Nóbrega | |
| Author | Sarno, Euzenir Nunes | |
| Author | Brennan, Patrick J. | |
| Author | Vissa, Varalakshmi D. | |
| Author | Suffys, Philip Noel | |
| Access date | 2018-09-25T17:24:17Z | |
| Available date | 2018-09-25T17:24:17Z | |
| Document date | 2009 | |
| Citation | FONTES, Amanda Nogueira Brum et al. Genetic diversity of mycobacterium leprae isolates from brazilian leprosy patients. Leprosy Review, v. 80, n. 3, p. 302-315, 2009. | |
| ISSN | 0305-7518 | |
| URI | https://www.arca.fiocruz.br/handle/icict/29051 | |
| Description | Produção científica do Laboratório de Biologia Molecular Aplicada a Micobactérias. | pt_BR |
| Description | Produção científica do Laboratório de Hanseníase. | pt_BR |
| Sponsorship | These studies were funded by NIH, NIAID grants AI-047197, AI-063457. | |
| Sponsorship | Contract NO1-AI-25469, by the Heiser Program for Research on Leprosy and Tuberculosis of the New York Community Trust through IDEAL – Initiative for Diagnostic and Epidemiological Assays for Leprosy. | |
| Sponsorship | CNPq (401283/2005-4). | |
| Sponsorship | Damien Foundation (ILEP). | |
| Language | eng | en_US |
| Publisher | Lepra | |
| Rights | open access | |
| Subject in Portuguese | Mycobacterium leprae | pt_BR |
| Subject in Portuguese | Hanseníase | pt_BR |
| Subject in Portuguese | Diversidade genética | pt_BR |
| Subject in Portuguese | Pacientes | pt_BR |
| Title | Genetic diversity of mycobacterium leprae isolates from brazilian leprosy patients | en_US |
| Type | Article | |
| DOI | 10.47276/lr.80.3.302 | |
| Abstract | Introduction: Leprosy is a chronic disease caused by infection with Mycobacterium leprae, an obligate intracellular parasite. A problem in studying the transmission of leprosy is the small amount of variation in bacterial genomic DNA. The discovery of variable number of tandem repeats (VNTRs) allowed the detection of strain variation in areas with a high prevalence of leprosy. Four genotypes of M. leprae based on three single-nucleotide polymorphism (SNPs) were also discovered to be useful for analysis of the global spread of leprosy. Methods: In this present study, we examined the allelic diversity of M. leprae at 16 select VNTR and three SNP loci using 89 clinical isolates obtained from patients mainly from the neighbouring states of São Paulo and Rio de Janeiro Brazil. Results and conclusion: By use of a PCR-RFLP-based procedure that allows the recognition of SNP types 3 and 4 without the need for the more expensive DNA sequencing steps, characterisation of the main M. leprae genotypes was easy. When applied on the study population, it was found that the SNP type 3 is most frequent in these two states of Brazil, and that VNTRs provided further discrimination of the isolates. Two Short Tandem Repeats (STRs) were monomorphic, with the remaining 14 STRs represented by two to 18 alleles. Epidemiological associations with, township or state were not evident in this random collection and require further investigations. In phylogenetic trees, branches formed by all 16 STRs clearly separated SNP type 3 organisms from the other types while the allelic patterns of two minisatellite loci 27-5 and 12-5 were highly correlated with SNP type 3. This strain typing study provide the basis for comparison of M. leprae strain types within Brazil and with those from other countries, and informed selection of genomic markers and methods for future studies. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular Aplicada a Micobactérias. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Colorado State University. Department of Microbiology, Immunology and Pathology. Fort Collins, CO, USA. | |
| Affilliation | Instituto Lauro de Souza Lima. Bauru. SP, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Colorado State University. Department of Microbiology, Immunology and Pathology. Fort Collins, CO, USA. | |
| Affilliation | Colorado State University. Department of Microbiology, Immunology and Pathology. Fort Collins, CO, USA. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular Aplicada a Micobactérias. Rio de Janeiro, RJ, Brasil. | |
| Subject | Mycobacterium leprae | en_US |
| Subject | Leprosy | en_US |
| Subject | Genetic diversity | en_US |
| Subject | Patients | en_US |
| e-ISSN | 2162-8807 | |
| xmlui.metadata.dc.subject.ods | 10 Redução das desigualdades | |
