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AutorZelazny, Adrian M.
AutorRoot, Jeremy M.
AutorShea, Yvonne R.
AutorColombo, Rhonda E.
AutorShamputa, Isdore C.
AutorStock, Frida
AutorConlan, Sean
AutorMcNulty, Steven
AutorBrown-Elliott, Barbara A.
AutorWallace, Richard J.
AutorOlivier, Kenneth N.
AutorHolland, Steven M.
AutorSampaio, Elizabeth P.
Fecha de acceso2018-09-25T18:04:55Z
Fecha de disponibilización2018-09-25T18:04:55Z
Fecha de publicación2009
ReferenciaZELAZNY, Adrian M. et al. Cohort Study of Molecular Identification and Typing of Mycobacterium abscessus, Mycobacterium massiliense, and Mycobacterium bolletii. Journal of Clinical Microbiology, v.47, n.7, p.1985-1995, July 2009.pt_BR
ISSN0095-1137pt_BR
URIhttps://www.arca.fiocruz.br/handle/icict/29054
Idiomaengpt_BR
EditorAmerican Society for Microbiologypt_BR
Derechos de autoropen access
Palabras clave en Portuguésidentificação molecularpt_BR
Palabras clave en Portuguésestudo de coortept_BR
Palabras clave en PortuguésMycobacterium abscessuspt_BR
Palabras clave en PortuguésMycobacterium massiliensept_BR
Palabras clave en PortuguésMycobacterium bolletiipt_BR
Palabras clave en Portuguésdoença pulmonarpt_BR
TítuloCohort study of molecular identification and typing of Mycobacterium abscessus, Mycobacterium massiliense, and Mycobacterium bolletiipt_BR
Tipo del documentoArticle
DOI10.1128/JCM.01688-08
Resumen en InglésMycobacterium abscessus is the most common cause of rapidly growing mycobacterial chronic lung disease. Recently, two new M. abscessus-related species, M. massiliense and M. bolletii, have been described. Health care-associated outbreaks have recently been investigated by the use of molecular identification and typing tools; however, very little is known about the natural epidemiology and pathogenicity of M. massiliense or M. bolletii outside of outbreak situations. The differentiation of these two species from M. abscessus is difficult and relies on the sequencing of one or more housekeeping genes. We performed extensive molecular identification and typing of 42 clinical isolates of M. abscessus, M. massiliense, and M. bolletii from patients monitored at the NIH between 1999 and 2007. The corresponding clinical data were also examined. Partial sequencing of rpoB, hsp65, and secA led to the unambiguous identification of 26 M. abscessus isolates, 7 M. massiliense isolates, and 2 M. bolletii isolates. The identification results for seven other isolates were ambiguous and warranted further sequencing and an integrated phylogenetic analysis. Strain relatedness was assessed by repetitive-sequence-based PCR (rep-PCR) and pulsed-field gel electrophoresis (PFGE), which showed the characteristic clonal groups for each species. Five isolates with ambiguous species identities as M. abscessus-M. massiliense by rpoB, hsp65, and secA sequencing clustered as a distinct group by rep-PCR and PFGE together with the M. massiliense type strain. Overall, the clinical manifestations of disease caused by each species were similar. In summary, a multilocus sequencing approach (not just rpoB partial sequencing) is required for division of M. abscessus and closely related species. Molecular typing complements sequence-based identification and provides information on prevalent clones with possible relevant clinical aspects.pt_BR
AfiliaciónNational Institutes of Health. Clinical Center. Department of Laboratory Medicine. Microbiology Service. Bethesda, Maryland, USA / National Institutes of Health. Clinical Center. Department of Laboratory Medicine. Immunopathogenesis Section. Bethesda, Maryland, USA.pt_BR
AfiliaciónNational Institutes of Health. Clinical Center. Department of Laboratory Medicine. Immunopathogenesis Section. Bethesda, Maryland, USA.pt_BR
AfiliaciónNational Institutes of Health. Clinical Center. Department of Laboratory Medicine. Microbiology Service. Bethesda, Maryland, USApt_BR
AfiliaciónNational Institutes of Health. Clinical Center. Department of Laboratory Medicine. Immunopathogenesis Section. Bethesda, Maryland, USA.pt_BR
AfiliaciónNational Institutes of Health. Clinical Center. Department of Laboratory Medicine. Tuberculosis Research Section. Bethesda, Maryland, USA.pt_BR
AfiliaciónNational Institutes of Health. Clinical Center. Department of Laboratory Medicine. Microbiology Service. Bethesda, Maryland, USApt_BR
AfiliaciónNational Institutes of Health. National Human Genome Research Institute. Bethesda, Maryland, USA.pt_BR
AfiliaciónThe University of Texas Health Science Center. Department of Microbiology. Tyler, Texas, USA.pt_BR
AfiliaciónThe University of Texas Health Science Center. Department of Microbiology. Tyler, Texas, USA.pt_BR
AfiliaciónThe University of Texas Health Science Center. Department of Microbiology. Tyler, Texas, USA.pt_BR
AfiliaciónNational Institutes of Health. Clinical Center. Department of Laboratory Medicine. Immunopathogenesis Section. Bethesda, Maryland, USA.pt_BR
AfiliaciónNational Institutes of Health. Clinical Center. Department of Laboratory Medicine. Immunopathogenesis Section. Bethesda, Maryland, USA / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.pt_BR
Palavras clave en InglêsCohort Studypt_BR
Palavras clave en Inglêsestudo de coortept_BR
Palavras clave en InglêsMycobacterium abscessuspt_BR
Palavras clave en InglêsMycobacterium massiliensept_BR
Palavras clave en InglêsMycobacterium bolletiipt_BR
Palavras clave en Inglêslung diseasept_BR
e-ISSN1098-660X
xmlui.metadata.dc.subject.ods03 Saúde e Bem-Estar


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