| Author | Fioramonte, Mariana | |
| Author | Jesus, Hugo Cesar Ramos de | |
| Author | Ferrari, Allan Jhonathan Ramos | |
| Author | Lima, Diogo Borges | |
| Author | Drekener, Roberta Lopes | |
| Author | Correia, Carlos Roque Duarte | |
| Author | Oliveira, Luciana Gonzaga | |
| Author | Ferreira, Ana Gisele da Costa Neves | |
| Author | Carvalho, Paulo Costa | |
| Author | Gozzo, Fabio Cesar | |
| Access date | 2018-09-27T13:19:04Z | |
| Available date | 2018-09-27T13:19:04Z | |
| Document date | 2018 | |
| Citation | FIORAMONTE, Mariana; et al. XPlex: An Effective, Multiplex Cross-Linking Chemistry for Acidic Residues. Anal. Chem., v.90, p.6043=6050, 2018. | pt_BR |
| ISSN | 0003-2700 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/29093 | |
| Language | eng | pt_BR |
| Publisher | American Chemical Society | pt_BR |
| Rights | restricted access | |
| Subject in Portuguese | X-Plex | pt_BR |
| Subject in Portuguese | Resíduos Ácidos | pt_BR |
| Subject in Portuguese | Química | pt_BR |
| Subject in Portuguese | Eficácia | pt_BR |
| Title | XPlex: An Effective, Multiplex Cross-Linking Chemistry for Acidic Residues | pt_BR |
| Type | Article | |
| DOI | 10.1021/acs.analchem.7b05135 | |
| Abstract | Cross-linking/Mass spectrometry (XLMS) is a consolidated technique for structural characterization of proteins and protein complexes. Despite its success, the cross-linking chemistry currently used is mostly based on N-hydroxysuccinimide (NHS) esters, which react primarily with lysine residues. One way to expand the current applicability of XLMS into several new areas is to increase the number of cross-links obtainable for a target protein. We introduce a multiplex chemistry (denoted XPlex) that targets Asp, Glu, Lys, and Ser residues. XPlex can generate significantly more cross-links with reactions occurring at lower temperatures and enables targeting proteins that are not possible with NHS ester-based cross-linkers. We demonstrate the effectiveness of our approach in model proteins as well as a target Lys-poor protein, SalBIII. Identification of XPlex spectra requires a search engine capable of simultaneously considering multiple cross-linkers on the same run; to achieve this, we updated the SIM-XL search algorithm with a search mode tailored toward XPlex. In summary, we present a complete chemistry/computational solution for significantly increasing the number of possible distance constraints by mass spectrometry experiments, and thus, we are convinced that XPlex poses as a real complementary approach for structural proteomics studies. | pt_BR |
| Affilliation | Universidade de Campinas. Instituto de Química. Campinas, SP, Brasil. | pt_BR |
| Affilliation | Universidade de Campinas. Instituto de Química. Campinas, SP, Brasil. | pt_BR |
| Affilliation | Universidade de Campinas. Instituto de Química. Campinas, SP, Brasil. | pt_BR |
| Affilliation | Institut Pasteur. Mass Spectometry for Biology Unit. Paris, France. | pt_BR |
| Affilliation | Universidade de Campinas. Instituto de Química. Campinas, SP, Brasil. | pt_BR |
| Affilliation | Universidade de Campinas. Instituto de Química. Campinas, SP, Brasil. | pt_BR |
| Affilliation | Universidade de Campinas. Instituto de Química. Campinas, SP, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Proteômica e Engenharia de Proteínas. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade de Campinas. Instituto de Química. Campinas, SP, Brasil. | pt_BR |
| Subject | X-Plex | pt_BR |
| Subject | Multiplex Cross-Linking Chemistry | pt_BR |
| Subject | Acid residues | pt_BR |
| Subject | efficiency | pt_BR |
| e-ISSN | 1520-6882 | |
| Embargo date | 2030-01-01 | |
| xmlui.metadata.dc.subject.ods | 12 Consumo e produção responsáveis | |
