| Author | Valente, Richard Hemmi | |
| Author | Sakai, Fernanda | |
| Author | Portes Junior, José Antonio | |
| Author | Viana, Luciana Godoy | |
| Author | Carneiro, Sylvia Mendes | |
| Author | Perales, Jonas | |
| Author | Yamanouye, Norma | |
| Access date | 2018-09-27T15:47:59Z | |
| Available date | 2018-09-27T15:47:59Z | |
| Document date | 2018 | |
| Citation | VALENTE, Richard Hemmi; et al. The Primary Duct of Bothrops jararaca Glandular Apparatus Secretes Toxins. Toxins, v.10, n.121, 13p, 2018. | pt_BR |
| ISSN | 2072-6651 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/29110 | |
| Language | eng | pt_BR |
| Publisher | MDPI | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Bothrops jararaca | pt_BR |
| Subject in Portuguese | aparelho glandular de veneno | pt_BR |
| Subject in Portuguese | duto primário | pt_BR |
| Subject in Portuguese | morfologia | pt_BR |
| Subject in Portuguese | ciclo secretor | pt_BR |
| Subject in Portuguese | produção de veneno | pt_BR |
| Subject in Portuguese | proteômica | pt_BR |
| Title | The Primary Duct of Bothrops jararaca Glandular Apparatus Secretes Toxins | pt_BR |
| Type | Article | |
| DOI | 10.3390/toxins10030121 | |
| Abstract | Despite numerous studies concerning morphology and venom production and secretion in the main venom gland (and some data on the accessory gland) of the venom glandular apparatus of Viperidae snakes, the primary duct has been overlooked. We characterized the primary duct of the Bothrops jararaca snake by morphological analysis, immunohistochemistry and proteomics. The duct has a pseudostratified epithelium with secretory columnar cells with vesicles of various electrondensities, as well as mitochondria-rich, dark, basal, and horizontal cells. Morphological analysis, at different periods after venom extraction, showed that the primary duct has a long cycle of synthesis and secretion, as do the main venom and accessory glands; however, the duct has a mixed mode venom storage, both in the lumen and in secretory vesicles. Mouse anti-B. jararaca venom serum strongly stained the primary duct's epithelium. Subsequent proteomic analysis revealed the synthesis of venom toxins-mainly C-type lectin/C-type lectin-like proteins. We propose that the primary duct's toxin synthesis products complement the final venom bolus. Finally, we hypothesize that the primary duct and the accessory gland (components of the venom glandular apparatus) are part of the evolutionary path from a salivary gland towards the main venom gland. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ. Brasil / Instituto Nacional de Ciência e Tecnologia em Toxinas. Brasília, DF, Brasil. | pt_BR |
| Affilliation | Instituto Butantan. Laboratório de Farmacologia. São Paulo, SP, Brasil. | pt_BR |
| Affilliation | Instituto Butantan. Laboratório de Imunopatologia. São Paulo, SP, Brasil. | pt_BR |
| Affilliation | Instituto Butantan. Laboratório de Farmacologia. São Paulo, SP, Brasil. | pt_BR |
| Affilliation | Instituto Butantan. Laboratório de Biologia Celular. São Paulo, SP, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ. Brasil / Instituto Nacional de Ciência e Tecnologia em Toxinas. Brasília, DF, Brasil. | pt_BR |
| Affilliation | Instituto Butantan. Laboratório de Farmacologia. São Paulo, SP, Brasil / Instituto Nacional de Ciência e Tecnologia em Toxinas. Brasília, DF, Brasil. | pt_BR |
| Subject | venom glandular apparatus | pt_BR |
| Subject | primary duct | pt_BR |
| Subject | morphology | pt_BR |
| Subject | secretory cycle | pt_BR |
| Subject | venom production | pt_BR |
| Subject | proteomics | pt_BR |
| Subject | Bothrops jararaca | pt_BR |
| e-ISSN | 2072-6651 | |
