Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/29300
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dc.contributor.authorBrasil, Pedro Emmanuel Alvarenga Americano do
dc.contributor.authorCastro, Rodolfo
dc.contributor.authorCastro, Liane de
dc.date.accessioned2018-10-03T17:49:55Z
dc.date.available2018-10-03T17:49:55Z
dc.date.issued2016
dc.identifier.citationDO BRASIL, P. E. A. A.; CASTRO, R.; CASTRO, L. Commercial enzyme-linked immunosorbent assay versus polymerase chain reaction for the diagnosis of chronic Chagas disease: a systematic review and meta-Analysis. Memórias do Instituto Oswaldo Cruz, v. 111, n. 1, p. 1-19, 2016.
dc.identifier.issn0074-0276
dc.identifier.urihttps://www.arca.fiocruz.br/handle/icict/29300
dc.language.isoeng
dc.rightsopen access
dc.titleCommercial enzyme-linked immunosorbent assay versus polymerase chain reaction for the diagnosis of chronic Chagas disease: a systematic review and meta-analysis
dc.typeArticle
dc.identifier.doi10.1590/0074-02760150296
dc.description.abstractenChronic Chagas disease diagnosis relies on laboratory tests due to its clinical characteristics. The aim of this research was to review commercial enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR) diagnostic test performance. Performance of commercial ELISA or PCR for the diagnosis of chronic Chagas disease were systematically searched in PubMed, Scopus, Embase, ISI Web, and LILACS through the bibliography from 1980-2014 and by contact with the manufacturers. The risk of bias was assessed with QUADAS-2. Heterogeneity was estimated with the I2 statistic. Accuracies provided by the manufacturers usually overestimate the accuracy provided by academia. The risk of bias is high in most tests and in most QUADAS dimensions. Heterogeneity is high in either sensitivity, specificity, or both. The evidence regarding commercial ELISA and ELISA-rec sensitivity and specificity indicates that there is overestimation. The current recommendation to use two simultaneous serological tests can be supported by the risk of bias analysis and the amount of heterogeneity but not by the observed accuracies. The usefulness of PCR tests are debatable and health care providers should not order them on a routine basis. PCR may be used in selected cases due to its potential to detect seronegative subjects.
dc.creator.affilliationFundação Oswaldo Cruz, Instituto Nacional de Infectologia Evandro Chagas, Laboratório de Pesquisa Clínica em Doença de Chagas, Rio de Janeiro, RJ, Brasil
dc.creator.affilliationFundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em DST/AIDS. Rio de Janeiro, RJ, Brasil./ Universidade Federal do Estado do Rio de Janeiro, Instituto de Saúde Coletiva, Rio de Janeiro, RJ, Brasil.
dc.creator.affilliationFundação Oswaldo Cruz, Instituto Nacional de Infectologia Evandro Chagas, Laboratório de Farmacogenética, Rio de Janeiro, RJ, Brasil
dc.subject.enChagas disease
dc.subject.enTrypanosoma cruzi
dc.subject.enDiagnosis
dc.subject.enPolymerase chain reaction
dc.subject.enEnzyme-linked immunosorbent assay
Appears in Collections:INI - Artigos de Periódicos



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