| Author | Borges, Tatiana Karla dos Santos | |
| Author | Alves, Érica Alessandra Rocha | |
| Author | Vasconcelos, Henda Aline da Rosa e | |
| Author | Carneiro, Fabiana Pirani | |
| Author | Nicola, André Moraes | |
| Author | Magalhães, Kelly Grace | |
| Author | Junqueira, Maria Imaculada Muniz | |
| Access date | 2018-10-05T18:17:05Z | |
| Available date | 2018-10-05T18:17:05Z | |
| Document date | 2017 | |
| Citation | BORGES, Tatiana Karla dos Santos et al. Differences in the modulation of reactive species, lipid bodies, cyclooxygenase-2, 5-lipoxygenase and PPAR-γ in cerebral malaria-susceptible and resistant mice. Immunobiology, v. 222, n. 4, p. 604-619, 2017. | pt_BR |
| ISSN | 0171-2985 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/29400 | |
| Language | eng | pt_BR |
| Publisher | Elsevier | pt_BR |
| Rights | restricted access | pt_BR |
| Subject in Portuguese | Eicosanóides | pt_BR |
| Subject in Portuguese | Peróxido de hidrogênio | pt_BR |
| Subject in Portuguese | Resposta inflamatória | pt_BR |
| Subject in Portuguese | Corpos lipídicos | pt_BR |
| Subject in Portuguese | Malaria | pt_BR |
| Subject in Portuguese | Óxido nítrico | pt_BR |
| Subject in Portuguese | PPAR-γ | pt_BR |
| Title | Differences in the modulation of reactive species, lipid bodies, cyclooxygenase-2, 5-lipoxygenase and PPAR-γ in cerebral malaria-susceptible and resistant mice | pt_BR |
| Type | Article | |
| DOI | 10.1016/j.imbio.2016.11.010 | |
| Abstract | Proinflammatory responses are associated with the severity of cerebral malaria. NO, H2O2, eicosanoid and PPAR-γ are involved in proinflammatory responses, but regulation of these factors is unclear in malaria. This work aimed to compare the expression of eicosanoid-forming-enzymes in cerebral malaria-susceptible CBA and C57BL/6 and -resistant BALB/c mice. Mice were infected with Plasmodium berghei ANKA, and the survival rates and parasitemia curves were assessed. On the sixth day post-infection, cyclooxygenase-2 and 5-lipoxygenase in brain sections were assessed by immunohistochemistry, and, NO, H2O2, lipid bodies, and PPAR-γ expression were assessed in peritoneal macrophages. The C57BL/6 had more severe disease with a lower survival time, higher parasitemia and lower production of plasmodicidal NO and H2O2 molecules than BALB/c. Enhanced COX-2 and 5-LOX expression were observed in brain tissue cells and vessels from C57BL/6 mice, and these mice expressed higher constitutive PPAR-γ levels. There was no translocation of PPAR-γ from cytoplasm to nucleus in macrophages from these mice. CBA mice had enhanced COX-2 expression in brain tissue cells and vessels and also lacked PPAR-γ cytoplasm-to-nucleus translocation. The resistant BALB/c mice presented higher survival time, lower parasitemia and higher NO and H2O2 production on the sixth day post-infection. These mice did not express either COX-2 or 5-LOX in brain tissue cells and vessels. Our data showed that besides the high parasite burden and lack of microbicidal molecules, an imbalance with high COX-2 and 5-LOX eicosanoid expression and a lack of regulatory PPAR-γ cytoplasm-to-nucleus translocation in macrophages were observed in mice that develop cerebral malaria. | pt_BR |
| Affilliation | Universidade de Brasilia. Faculdade de Medicina. Laboratório de Imunologia Celular. Laboratório de Imunologia Celular. Brasilia, DF, Brazil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Laboratorio de Imunologia Molecular e Celular. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Universidade de Brasilia. Faculdade de Medicina. Laboratório de Imunologia Celular. Laboratório de Imunologia Celular. Brasilia, DF, Brazil / National Direction of Public Health. Ministry of Health of the Republic of Angola. Luanda, Angola. | pt_BR |
| Affilliation | Universidade de Brasília. Faculdade de Medicina. Laboratório de Patologia. Brasilia, DF, Brazil. | pt_BR |
| Affilliation | Universidade de Brasilia. Faculdade de Medicina. Laboratório de Imunologia Celular. Laboratório de Imunologia Celular. Brasilia, DF, Brazil. | pt_BR |
| Affilliation | Universidade de Brasília. Instituto de Biologia. Departamento de Biologia Celular. Laboratório de Imunologia e Inflamação. Brasilia, DF, Brazil. | pt_BR |
| Affilliation | Universidade de Brasilia. Faculdade de Medicina. Laboratório de Imunologia Celular. Laboratório de Imunologia Celular. Brasilia, DF, Brazil. | pt_BR |
| Subject | Eicosanoids | pt_BR |
| Subject | Hydrogen peroxide | pt_BR |
| Subject | Inflammatory response | pt_BR |
| Subject | Lipid bodies | pt_BR |
| Subject | Malaria | pt_BR |
| Subject | Nitric oxide | pt_BR |
| Subject | PPAR-γ | pt_BR |
| Embargo date | 2025-01-01 | |
