| Author | Nicolau, Carolina Alves | |
| Author | Prorock, Alyson | |
| Author | Bao, Yongde | |
| Author | Ferreira, Ana Gisele da Costa Neves | |
| Author | Valente, Richard Hemmi | |
| Author | Fox, Jay William | |
| Access date | 2018-10-05T18:34:49Z | |
| Available date | 2018-10-05T18:34:49Z | |
| Document date | 2018 | |
| Citation | NICOLAU, Carolina Alves; et al. Revisiting the Therapeutic Potential of Bothrops jararaca Venom: Screening for Novel Activities Using Connectivity Mapping. Toxins, v.10, n.69, 26p, 2018. | pt_BR |
| ISSN | 2072-6651 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/29403 | |
| Language | eng | pt_BR |
| Publisher | MDPI | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Bothrops jararaca | pt_BR |
| Subject in Portuguese | potencial terapêutico | pt_BR |
| Subject in Portuguese | mapa de conectividade | pt_BR |
| Subject in Portuguese | descoberta de drogas | pt_BR |
| Subject in Portuguese | medicamentos biossimilares | pt_BR |
| Title | Revisiting the Therapeutic Potential of Bothrops jararaca Venom: Screening for Novel Activities Using Connectivity Mapping | pt_BR |
| Type | Article | |
| DOI | 10.3390/toxins10020069 | |
| Abstract | Snake venoms are sources of molecules with proven and potential therapeutic applications. However, most activities assayed in venoms (or their components) are of hemorrhagic, hypotensive, edematogenic, neurotoxic or myotoxic natures. Thus, other relevant activities might remain unknown. Using functional genomics coupled to the connectivity map (C-map) approach, we undertook a wide range indirect search for biological activities within the venom of the South American pit viper Bothrops jararaca. For that effect, venom was incubated with human breast adenocarcinoma cell line (MCF7) followed by RNA extraction and gene expression analysis. A list of 90 differentially expressed genes was submitted to biosimilar drug discovery based on pattern recognition. Among the 100 highest-ranked positively correlated drugs, only the antihypertensive, antimicrobial (both antibiotic and antiparasitic), and antitumor classes had been previously reported for B. jararaca venom. The majority of drug classes identified were related to (1) antimicrobial activity; (2) treatment of neuropsychiatric illnesses (Parkinson's disease, schizophrenia, depression, and epilepsy); (3) treatment of cardiovascular diseases, and (4) anti-inflammatory action. The C-map results also indicated that B. jararaca venom may have components that target G-protein-coupled receptors (muscarinic, serotonergic, histaminergic, dopaminergic, GABA, and adrenergic) and ion channels. Although validation experiments are still necessary, the C-map correlation to drugs with activities previously linked to snake venoms supports the efficacy of this strategy as a broad-spectrum approach for biological activity screening, and rekindles the snake venom-based search for new therapeutic agents. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia em Toxinas. Brasília, DF, Brasil / University of Virginia. Department of Microbiology, Immunology and Cancer Biology. Charlottesville, VA, USA. | pt_BR |
| Affilliation | University of Virginia. Department of Microbiology, Immunology and Cancer Biology. Charlottesville, VA, USA. | pt_BR |
| Affilliation | University of Virginia. Department of Microbiology, Immunology and Cancer Biology. Charlottesville, VA, USA. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia em Toxinas. Brasília, DF, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia em Toxinas. Brasília, DF, Brasil. | pt_BR |
| Affilliation | University of Virginia. Department of Microbiology, Immunology and Cancer Biology. Charlottesville, VA, USA. | pt_BR |
| Subject | Bothrops jararaca | pt_BR |
| Subject | therapeutic potential | pt_BR |
| Subject | connectivity map | pt_BR |
| Subject | drug discovery | pt_BR |
| Subject | biosimilar drugs | pt_BR |
| e-ISSN | 2072-6651 | |
| xmlui.metadata.dc.subject.ods | 03 Saúde e Bem-Estar | |
