| Author | Salvio, A. L. | |
| Author | Lopes, A. G. | |
| Author | Almeida, A. J. | |
| Author | Gardinali, N. R. | |
| Author | LIma, L. R. P. | |
| Author | Oliveira, J. M. de | |
| Author | Sion, F. S. | |
| Author | Ribeiro, L. C. P. | |
| Author | Pinto, M. A. | |
| Author | Paula, V. S. de | |
| Access date | 2018-10-18T17:29:28Z | |
| Available date | 2018-10-18T17:29:28Z | |
| Document date | 2018 | |
| Citation | SALVIO, A. L. et al. Detection and quantification of hepatitis E virus in the absence of IgG and IgM anti-HEV in HIV-positive patients. Journal of Applied Microbiology, v. 125, p. 1208-1215, 2018. | pt_BR |
| ISSN | 1364-5072 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/29635 | |
| Language | eng | pt_BR |
| Publisher | Wiley | pt_BR |
| Rights | restricted access | |
| Subject in Portuguese | Hepatite E | pt_BR |
| Subject in Portuguese | Diagnóstico | pt_BR |
| Subject in Portuguese | HIV | pt_BR |
| Subject in Portuguese | Co-ínfecção | pt_BR |
| Subject in Portuguese | Reação em Cadeia da Polimerase em Tempo Real | pt_BR |
| Subject in Portuguese | Carga vital | pt_BR |
| Subject in Portuguese | Curva padrão | pt_BR |
| Title | Detection and quantification of hepatitis E virus in the absence of IgG and IgM anti-HEV in HIV-positive patien | pt_BR |
| Type | Article | |
| DOI | 10.1111/jam.14024 | |
| Abstract | Aims: To improve RT-qPCR with an internal control and a synthetic standard
curve to detect HEV in HIV co-infected patients.
Methods and Results: A single-stranded RNA (ssRNA) and a double-stranded
DNA (dsDNA) synthetic curve were designed, compared to the international
reference panel for HEV genotypes, and tested to quantify and detect a
reference panel for HEV genotypes. The detection limit of the RNA synthetic
curve (50 copies per ml) was better than the DNA synthetic curve (100 copies
per ml) and the WHO standard curve (250 copies per ml). Then, 280 serum
samples from HIV-positive patients were tested for HEV RNA, which was
detected in 3 6% of serum samples. The viral load ranged from 2 9 102 copies
per ml to 4 78 9 108 copies per ml. HEV IgM/IgG antibodies were not
detected in the RNA-positive patients. Sequencing analysis of HEV showed that
the virus belongs to genotype 3 (HEV GT3).
Conclusions: Real-time PCR was a useful tool to estimate co-infection with
HEV/HIV, even in patients with low viral loads and undetectable anti-HEV
IgG and IgM antibodies.
Significance and Impact of the Study: Hepatitis E virus genotype 3 (HEV
GT3) has been associated with silent chronic hepatitis and cirrhosis in HIVpositive
subjects worldwide, but there is a lack of data on this co-infection in
Brazil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Molecular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Molecular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Estado do Rio de Janeiro. Hospital Universitário Gaffreé Guinle. Departamento de Hematologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Desenvolvimento Tecnológico em Virologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Molecular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Desenvolvimento Tecnológico em Virologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Estado do Rio de Janeiro. Hospital Universitário Gaffreé Guinle. Departamento de Hematologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Estado do Rio de Janeiro. Hospital Universitário Gaffreé Guinle. Departamento de Hematologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Desenvolvimento Tecnológico em Virologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Molecular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Coinfection | pt_BR |
| Subject | Diagnosis | pt_BR |
| Subject | Hepaitits E | pt_BR |
| Subject | HIV | pt_BR |
| Subject | Real time PCR | pt_BR |
| Subject | Standard curve | pt_BR |
| Subject | Vital load | pt_BR |
| DeCS | Reação em Cadeia da Polimerase em Tempo Real | pt_BR |
| e-ISSN | 1365-2672 | |
| Embargo date | 2030-01-01 | |
