Author | Raposo, Ana Varela | |
Author | Pimentela, Catarina | |
Author | Silva, Fabio Morais | |
Author | Rezende, Antonio | |
Author | Ruiz, Jerônimo Conceição | |
Author | Pousada, Claudina Rodrigues | |
Access date | 2018-10-29T17:44:41Z | |
Available date | 2018-10-29T17:44:41Z | |
Document date | 2013 | |
Citation | RAPOSO, Ana Varela et al. Role of NorR-like transcriptional regulators under nitrosative stress of the δ-proteobacterium, Desulfovibrio gigas. Biochemical and Biophysical Research Communications, v. 431, n. 3, p. 590-596, 2013 | pt_BR |
ISSN | 0006-291X | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/29791 | |
Language | eng | pt_BR |
Publisher | Elsevier | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | D. gigas | pt_BR |
Subject in Portuguese | Estresse Nitrosativo | pt_BR |
Subject in Portuguese | NorR-like | pt_BR |
Subject in Portuguese | Fatores de transcrição | pt_BR |
Subject in Portuguese | Análise filogenética | pt_BR |
Title | Role of NorR-like transcriptional regulators under nitrosative stress of the δ-proteobacterium, Desulfovibrio gigas. | pt_BR |
Type | Article | pt_BR |
DOI | 10.1016/j.bbrc.2012.12.130 | |
Abstract | NorR protein was shown to be responsible for the transcriptional regulation of flavorubredoxin and its associated oxidoreductase in Escherichia coli. Since Desulfovibrio gigas has a rubredoxin:oxygen oxidoreductase (ROO) that is involved in both oxidative and nitrosative stress response, a NorR-like protein was searched in D. gigas genome. We have found two putative norR coding units in its genome. To study the role of the protein designated as NorR1-like (NorR1L) in the presence of nitrosative stress, a norR1L null mutant of D. gigas was created and a phenotypic analysis was performed under the nitrosating agent GSNO. We show that under these conditions, the growth of both D. gigas mutants Δroo and ΔnorR1-like is impaired. In order to confirm that D. gigas NorR1-like may play identical function as the NorR of E. coli, we have complemented the E. coli ΔnorR mutant strain with the norR1-like gene and have evaluated growth when nitrosative stress was imposed. The growth phenotype of E. coli ΔnorR mutant strain was recovered under these conditions. We also found that induction of roo gene expression is completely abolished in the norR1L mutant strain of D. gigas subjected to nitrosative stress. It is identified in δ-proteobacteria, for the first time a transcription factor that is involved in nitrosative stress response and regulates the rd-roo gene expression. | pt_BR |
Affilliation | Universidade Nova de Lisboa. Instituto de Tecnologia Química e Biológica. Oeiras, Portugal | pt_BR |
Affilliation | Universidade Nova de Lisboa. Instituto de Tecnologia Química e Biológica. Oeiras, Portugal | pt_BR |
Affilliation | Universidade Nova de Lisboa. Instituto de Tecnologia Química e Biológica. Oeiras, Portugal | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa René Rachou. Laboratório de Parasitologia Celular e Molecular. Belo Horizonte, MG, Brazil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa René Rachou. Laboratório de Parasitologia Celular e Molecular. Belo Horizonte, MG, Brazil | pt_BR |
Affilliation | Universidade Nova de Lisboa. Instituto de Tecnologia Química e Biológica. Oeiras, Portugal | pt_BR |
Subject | D. gigas | pt_BR |
Subject | Nitrosative stress | pt_BR |
Subject | NorR-like | pt_BR |
Subject | Transcription factors | pt_BR |
Subject | Phylogenetic analysis | pt_BR |
Embargo date | 2020-01-01 | |