| Author | Cunha, Ananda de Castro | |
| Author | Chierrito, Talita Perez Cantuaria | |
| Author | Machado, Gerzia Maria de Carvalho | |
| Author | Leon, Leonor Laura Pinto | |
| Author | Silva, Cleuza Conceição da | |
| Author | Tanaka, Julio Cesar | |
| Author | Souza, Lauro Mera de | |
| Author | Gonçalves, Regina Aparecida Correia | |
| Author | Oliveira, Arildo José Braz de | |
| Access date | 2018-11-06T11:19:16Z | |
| Available date | 2018-11-06T11:19:16Z | |
| Document date | 2012 | |
| Citation | CUNHA, Ananda de Castro; et al. Anti-leishmanial activity of alkaloidal extracts obtained from different organs of Aspidosperma ramiflorum. Phytomedicine, v.19, p.413– 417, 2012. | pt_BR |
| ISSN | 0944-7113 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/29879 | |
| Language | eng | pt_BR |
| Publisher | Elsevier | pt_BR |
| Rights | restricted access | |
| Subject in Portuguese | Leishmania | pt_BR |
| Subject in Portuguese | Aspidosperma ramiflorum | pt_BR |
| Subject in Portuguese | Alcalóides indólicos monoterpênicos | pt_BR |
| Title | Anti-leishmanial activity of alkaloidal extracts obtained from different organs of Aspidosperma ramiflorum | pt_BR |
| Type | Article | |
| DOI | 10.1016/j.phymed.2011.12.004 | |
| Abstract | The present study was designated to evaluate semi-quantitative antileishmanial activity of alkaloidal
extracts that were obtained from 1 g of different parts of Aspidosperma ramiflorum (leaves, roots, seeds,
and stem barks). Alkaloidal extracts of barks and leaves presented a good activity against the extracellular
form (promastigotes) of Leishmania (L.) amazonensis. It is known that compounds responsible for
the antileishmanial activity in the alkaloidal extracts from A. ramiflorum are the monoterpenoid indole
alkaloids ramiflorine A and ramiflorine B, therefore extracts obtained from different plant parts were
analyzed by electrospray ionization mass spectrometry (ESI-MS) in order to evidence the presence of
these bioactive alkaloids. Based on these findings, alkaloidal extract from leaves was fractionated on
preparative thin-layer chromatography in a bioassay-guided fractionation affording individual purified
ramiflorines A and B. Both ramiflorines A and B showed significant activity against Leishmania (L.) amazonensis
(LD50 values of 18.5
±
6.5 g/ml and 12.63
±
5.52 g/ml, respectively). Our results are showing
that alkaloidal extract from leaves is a promising alternative to the use of stem barks from A. ramiflorum. | pt_BR |
| Affilliation | Universidade Estadual de Maringá. Departamento de Farmácia. Maringá, PR, Brasil. | pt_BR |
| Affilliation | Universidade Estadual de Maringá. Departamento de Farmácia. Maringá, PR, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Imunologia. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Imunologia. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Universidade Estadual de Maringá. Departamento de Química. Maringá, PR, Brasil. | pt_BR |
| Affilliation | Universidade Estadual de Maringá. Departamento de Química. Maringá, PR, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Paraná. Centro Politécnico. Departamento de Bioquímica e Biologia Molecular. Curitiba, PR, Brasil | pt_BR |
| Affilliation | Universidade Estadual de Maringá. Departamento de Farmácia. Maringá, PR, Brasil. | pt_BR |
| Affilliation | Universidade Estadual de Maringá. Departamento de Farmácia. Maringá, PR, Brasil. | pt_BR |
| Subject | Leishmania | pt_BR |
| Subject | Aspidosperma ramiflorum | pt_BR |
| Subject | Monoterpenoid indole alkaloids | pt_BR |
| e-ISSN | 1618-095X | |
| Embargo date | 2030-01-01 | |
