| Author | Inacio, Job D. F. | |
| Author | Canto-Carvalheiro, Marilene M. | |
| Author | Menna-Barreto, Rubem F. S. | |
| Author | Amaral, Elmo E. Almeida | |
| Access date | 2018-11-06T17:12:14Z | |
| Available date | 2018-11-06T17:12:14Z | |
| Document date | 2012 | |
| Citation | INACIO, Job D. F.; CAVALHEIRO, Marilene M. Canto; AMARAL, MENNA-BARRETO, Rubem F. S.; AMARAL, Elmo E. Almeida. Mitochondrial damage contribute to epigallocatechin-3-gallate induced death in Leishmania amazonensis . Experimental Parasitology, v.132, p.151-155, June 2012. | pt_BR |
| ISSN | 0014-4894 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/29909 | |
| Language | eng | pt_BR |
| Publisher | Elsevier | pt_BR |
| Rights | restricted access | |
| Subject in Portuguese | Mitocôndrias | pt_BR |
| Subject in Portuguese | Leishmania amazonensis | pt_BR |
| Subject in Portuguese | flavonóide | pt_BR |
| Title | Mitochondrial damage contribute to epigallocatechin-3-gallate induced death in Leishmania amazonensis | pt_BR |
| Type | Article | |
| DOI | 10.1016/j.exppara.2012.06.008 | |
| Abstract | Epigallocatechin-3-gallate (EGCG), the most abundant flavonoid in green tea, has been reported to have
antiproliferative effects on Trypanosoma cruzi however, the mechanism of protozoan action of EGCG has
not been studied. In the present study, we demonstrate the mechanism for the antileishmanial activity of
EGCG against Leishmania amazonensis promastigotes. Incubation with EGCG significantly inhibited L.
amazonensis promastigote proliferation in a time- and dose-dependent manner. The IC50 for EGCG at
120 h was 0.063 mM. Ultrastructural alterations of the mitochondria were observed in promastigote treated
with EGCG, being the organelle injury reinforced by the decrease in rhodamine 123 fluorescence. The
effects of several drugs that interfere directly with mitochondrial physiology in parasites such as Leishmania
have been described. The unique mitochondrial features of Leishmania make this organelle an ideal
drug target while minimizing toxicity. These data suggest mitochondrial collapse as a part of the EGCG
mechanism of action and demonstrate the leishmanicidal effect of EGCG. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica de Tripanosomatideos. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica de Tripanosomatideos. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Celular. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica de Tripanosomatideos. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Subject | Mitochondrial collapse | pt_BR |
| Subject | Mitochondrial membrane potential (Dwm) | pt_BR |
| Subject | pigallocatechin-3-gallate | pt_BR |
| Subject | Mitochondria | pt_BR |
| Subject | Flavonoid | pt_BR |
| Subject | Leishmania amazonensis | pt_BR |
| DeCS | Arilsulfotransferase | pt_BR |
| DeCS | Potencial da Membrana Mitocondrial | pt_BR |
| e-ISSN | 1090-2449 | |
| Embargo date | 2030-01-01 | |
