| Author | Gomes, Tatiana Luna | |
| Author | Magalhães, Kelly G. | |
| Author | Santos, Fabio P. Mesquita | |
| Author | Abreu, Ilka Bakker | |
| Author | Samico, Rafaela F. | |
| Author | Molinaro, Raphael | |
| Author | Calheiros, Andrea S. | |
| Author | Diaz, Bruno L. | |
| Author | Bozza, Patrícia T. | |
| Author | Weller, Peter F. | |
| Author | Melo, Christianne Bandeira | |
| Access date | 2018-11-15T13:17:45Z | |
| Available date | 2018-11-15T13:17:45Z | |
| Document date | 2011 | |
| Citation | GOMES, Tatiana Luna; et al. Eosinophils as a novel cell source of prostaglandin D2: autocrine role in allergic inflammation. J. Immunol., v.187, n.12, p.6518-6526, Dec. 2011. | pt_BR |
| ISSN | 0022-1767 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/30037 | |
| Language | eng | pt_BR |
| Publisher | American Association of Immunologists | pt_BR |
| Rights | restricted access | |
| Subject in Portuguese | eosinófilos | pt_BR |
| Subject in Portuguese | mastócitos | pt_BR |
| Subject in Portuguese | leucotrienos cisteínicos | pt_BR |
| Subject in Portuguese | alergia | pt_BR |
| Subject in Portuguese | gotículas lipídicas | pt_BR |
| Title | Eosinophils as a novel cell source of prostaglandin D2: autocrine role in allergic inflammation | pt_BR |
| Type | Article | |
| DOI | 10.4049/jimmunol.1101806 | |
| Abstract | PGD(2) is a key mediator of allergic inflammatory diseases that is mainly synthesized by mast cells, which constitutively express high levels of the terminal enzyme involved in PGD(2) synthesis, the hematopoietic PGD synthase (H-PGDS). In this study, we investigated whether eosinophils are also able to synthesize, and therefore, supply biologically active PGD(2). PGD(2) synthesis was evaluated within human blood eosinophils, in vitro differentiated mouse eosinophils, and eosinophils infiltrating inflammatory site of mouse allergic reaction. Biological function of eosinophil-derived PGD(2) was studied by employing inhibitors of synthesis and activity. Constitutive expression of H-PGDS was found within nonstimulated human circulating eosinophils. Acute stimulation of human eosinophils with A23187 (0.1-5 μM) evoked PGD(2) synthesis, which was located at the nuclear envelope and was inhibited by pretreatment with HQL-79 (10 μM), a specific H-PGDS inhibitor. Prestimulation of human eosinophils with arachidonic acid (10 μM) or human eotaxin (6 nM) also enhanced HQL-79-sensitive PGD(2) synthesis, which, by acting on membrane-expressed specific receptors (D prostanoid receptors 1 and 2), displayed an autocrine/paracrine ability to trigger leukotriene C(4) synthesis and lipid body biogenesis, hallmark events of eosinophil activation. In vitro differentiated mouse eosinophils also synthesized paracrine/autocrine active PGD(2) in response to arachidonic acid stimulation. In vivo, at late time point of the allergic reaction, infiltrating eosinophils found at the inflammatory site appeared as an auxiliary PGD(2)-synthesizing cell population. Our findings reveal that eosinophils are indeed able to synthesize and secrete PGD(2), hence representing during allergic inflammation an extra cell source of PGD(2), which functions as an autocrine signal for eosinophil activation. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Inflamação. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Inflamação. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Inflamação. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Inflamação. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Inflamação. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Harvard Medical School. Beth Israel Deaconess Medical Center. Department of Medicine. Division of Allergy and Inflammation. Boston, MA, USA. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Inflamação. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | PGD2 | pt_BR |
| Subject | eosinophils | pt_BR |
| Subject | mast cells | pt_BR |
| Subject | cysteinyl leukotrienes | pt_BR |
| Subject | allergy | pt_BR |
| Subject | lipid droplets | pt_BR |
| Subject | CCL11 | pt_BR |
| e-ISSN | 1550-6606 | |
| Embargo date | 2030-01-01 | |
