| Author | Rodrigues, Ana Claudia Souza | |
| Author | Chang, Marilene Rodrigues | |
| Author | Nóbrega, Gabriela Dorn | |
| Author | Rodrigues, Mayara Souza | |
| Author | Carvalho, Nadia Cristina Pereira | |
| Author | Gomes, Beatriz Garcia | |
| Author | Almeida, Dênio Lopes de | |
| Author | Carvalho, Karyne Rangel | |
| Author | Asensi, Marise Dutra | |
| Access date | 2018-11-18T16:27:22Z | |
| Available date | 2018-11-18T16:27:22Z | |
| Document date | 2011 | |
| Citation | RODRIGUES, Ana Claudia Souza; et al. Metallo-β-lactamase and genetic diversity of Pseudomon.as aeruginosa in intensive care units in Campo Grande, MS, Brazil. Braz J Infect Dis, v.15, n.3, p.195-199. 2011. | pt_BR |
| ISSN | 1413-8670 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/30075 | |
| Language | eng | pt_BR |
| Publisher | Elsevier | pt_BR |
| Rights | restricted access | |
| Subject in Portuguese | Pseudomonas aeruginosa | pt_BR |
| Subject in Portuguese | Múltipla ressitência a drogas | pt_BR |
| Subject in Portuguese | eletroforese | pt_BR |
| Subject in Portuguese | gel | pt_BR |
| Subject in Portuguese | campo pulsado | pt_BR |
| Title | Metallo-β-lactamase and genetic diversity of Pseudomonas aeruginosa in intensive care units in Campo Grande, MS, Brazil | pt_BR |
| Type | Article | |
| Abstract | Infection by Pseudomonas aeruginosa has spread worldwide, with limited options for treatment. The purpose of this study was to investigate metallo-β-lactamase-producing P. aeruginosa strains and compare their genetic profile using samples collected from patients in intensive care units. Forty
P. aeruginosa strains were isolated from two public hospitals in Campo Grande, Mato Grosso do Sul State, from January 1st, 2007 to June 31st, 2008. Profiles of antimicrobial susceptibility were determined using the agar diffusion method. Metallo-β-lactamase was investigated using the double-disk diffusion test and PCR. Molecular typing was performed by pulsed-field gel electrophoresis (PFGE). Respiratory and urinary tracts were the most common isolation sites. Of the 40 samples tested, 72.5% (29/40) were resistant to ceftazidime and 92.5% (37/40) to imipenem, whereas 65% (26/40) were resistant to both antimicrobials. Fifteen pan-resistant samples were found. Five percent (2/40) of samples were positive for metallo-β-lactamase on the phenotype test. No metallo-β-lactamase subtype was detected by PCR. Macrorestriction analysis revealed 14 distinct genetic patterns. Based on the superior accuracy of PCR, it can be inferred that P. aeruginosa isolates from the investigated hospitals have alternative mechanisms of carbapenem resistance. The results also suggest clonal spread of
P. aeruginosa between the studied hospitals. | pt_BR |
| Affilliation | Universidade Federal de Mato Grosso do Sul. Programa de Pós-Graduação em Saúde e Desenvolvimento. Campo Grande,, MS, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Mato Grosso do Sul. Departamento de Farmácia e Bioquímica. Campo Grande, MS, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Mato Grosso do Sul. Departamento de Farmácia e Bioquímica. Campo Grande, MS, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Mato Grosso do Sul. Departamento de Farmácia e Bioquímica. Campo Grande, MS, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Mato Grosso do Sul. Hospital Universitário. Campo Grande, MS, Brasil. | pt_BR |
| Affilliation | Hospital Regional de Mato Grosso do Sul. MS, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Mato Grosso do Sul. Departamento de Farmácia e Bioquímica. Campo Grande, MS, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Infecção Hospitalar. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Infecção Hospitalar. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Pseudomonas aeruginosa | pt_BR |
| Subject | drug resistance, multiple | pt_BR |
| Subject | beta-lactamases | pt_BR |
| Subject | electrophoresis | pt_BR |
| Subject | gel | pt_BR |
| Subject | pulsed-field | pt_BR |
| e-ISSN | 1678-4391 | |
| Embargo date | 2030-01-01 | |
