| Author | Pinto, Mauro Cunha Xavier | |
| Author | Cota, Betania Barros | |
| Author | Rodrigues, Michele A. | |
| Author | Leite, Maria Fatima | |
| Author | Fagundes, Elaine Maria Souza | |
| Access date | 2018-11-19T12:11:55Z | |
| Available date | 2018-11-19T12:11:55Z | |
| Document date | 2013 | |
| Citation | PINTO, Mauro Cunha Xavier et al. The Cytotoxic and Proapoptotic Activities of Hypnophilin are Associated with Calcium Signaling in UACC-62 Cells. J Biochem Mol Toxicol., v. 27, n. 11, p. 479-485, 2013 | pt_BR |
| ISSN | 1095-6670 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/30086 | |
| Language | eng | pt_BR |
| Publisher | Wiley | pt_BR |
| Rights | restricted access | pt_BR |
| Subject in Portuguese | produtos naturais | pt_BR |
| Subject in Portuguese | celulas cancerigenas | pt_BR |
| Title | The Cytotoxic and Proapoptotic Activities of Hypnophilin are Associated with Calcium Signaling in UACC-62 Cells | pt_BR |
| Type | Article | pt_BR |
| DOI | 10.1002/jbt.21507 | |
| Abstract | Hypnophilin (HNP) is a sesquiterpene that is isolated from Lentinus cf. strigosus and has cytotoxic activities. Here, we studied the calcium signaling and cytotoxic effects ofHNPinUACC-62cells, ahuman skin melanoma cell line. HNP was able to increase the intracellular calcium concentration in UACC-62 cells, which was blocked in cells stimulated in Ca2+-freemedia. HNP treatment with BAPTA-AM, an intracellular Ca2+ chelator, caused an increase in calcium signals. HNP showed cytotoxicity against UACC-62 cells in which it induced DNA fragmentation and morphological alterations, including changes in the nuclear chromatin profile and increased cytoplasmatic vacuolization, but it had no effect on the plasma membrane integrity. These data suggest that cytotoxicity in UACC-62 cells, after treatment with HNP, is associated with Ca2+ influx. Together, these findings suggest that HNP is a relevant tool for the further investigation of new anticancer approaches. | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Departamento de Fisiologia e Biofısica. Belo Horizonte, MG, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Laboratorio de Quımica de Produtos Naturais. Belo Horizonte, MG, Brasil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Departamento de Fisiologia e Biofısica. Belo Horizonte, MG, Brasil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Departamento de Fisiologia e Biofısica. Belo Horizonte, MG, Brasil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Departamento de Fisiologia e Biofısica. Belo Horizonte, MG, Brasil | pt_BR |
| Subject | Natural Products | pt_BR |
| Subject | Cancer Cells | pt_BR |
| Subject | Apoptosis | pt_BR |
| Subject | Calcium Signaling | pt_BR |
| Embargo date | 2100-01-01 | |
