Author | Stahl, Philipp | |
Author | Ruppert, Volker | |
Author | Meyer, Thomas | |
Author | Schmidt, Jorg | |
Author | Campos, Marco Antonio | |
Author | Gazzinelli, Ricardo Tostes | |
Author | Maisch, Bernhard | |
Author | Schwarz, Ralph T. | |
Author | Debierre-Grockiego, Francoise | |
Access date | 2018-11-26T16:06:06Z | |
Available date | 2018-11-26T16:06:06Z | |
Document date | 2013 | |
Citation | STAHL, Philipp et al. Trypomastigotes and amastigotes of Trypanosoma cruzi induce apoptosis and STAT3 activation in cardiomyocytes in vitro. Apoptosis., v. 18, n. 6, p. 653-63, 2013 | pt_BR |
ISSN | 1360-8185 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/30195 | |
Language | eng | pt_BR |
Publisher | Springer | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Trypanosoma cruzi | pt_BR |
Subject in Portuguese | tripomastigota | pt_BR |
Subject in Portuguese | Amastigota | pt_BR |
Subject in Portuguese | Cardiomiócito | pt_BR |
Title | Trypomastigotes and amastigotes of Trypanosoma cruzi induce apoptosis and STAT3 activation in cardiomyocytes in vitro. | pt_BR |
Type | Article | pt_BR |
DOI | 10.1007/s10495-013-0822-x | |
Abstract | The haemoflagellate Trypanosoma cruzi is the causative agent of Chagas' disease that occurs in approximately 8 million people in Latin America. Patients infected with T. cruzi frequently suffer of cardiomegaly and may die of myocardial failure. Here we show that T. cruzi trypomastigotes (extracellular form) increased in vitro apoptosis of rat cardiomyocytes. Additionally, we demonstrated that amastigotes (intracellular form), for which a method for purification was established, were also able to induce cardiomyocyte apoptosis. Increase of apoptosis was associated with up-regulation of the apoptotic gene bax by trypomastigotes, while expression of the anti-apoptotic gene bcl-2 was down-regulated by amastigotes. The transcription factor STAT3 but not STAT1 was activated in cardiomyocytes by trypomastigotes. In addition, tlr7 gene expression was up-regulated in cardiomyocytes incubated with trypomastigotes, suggesting that this Toll-like receptor is involved in the intracellular recognition after host cell invasion by T. cruzi. Glycosylphosphatidylinositols purified from trypomastigotes did not induce cardiomyocyte apoptosis and STAT activation but down-regulated tlr7 gene expression. In conclusion, cardiomyopathy observed in Chagas' disease might be in part due to apoptosis of cardiomyocytes induced directly by the parasite. | pt_BR |
Affilliation | Laboratory of Parasitology. Institute for Virology. Philipps University. Marburg, Germany | pt_BR |
Affilliation | Department of Cardiology, Philipps University, Marburg, Germany/Department of Cardiology, Philipps University, Marburg, Germany | pt_BR |
Affilliation | Department of Psychosomatic Medicine and Psychotherapy, Georg August University, 37075 Gottingen, Germany/ Department of Cardiology, Philipps University, Marburg, Germany | pt_BR |
Affilliation | Laboratory of Parasitology. Institute for Virology. Philipps University. Marburg, Germany | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Rene Rachou. Belo Horizonte, MG, Brazil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Rene Rachou. Belo Horizonte, MG, Brazil/Division of Infectious Diseases and Immunology. Department of Medicine. University of Massachusetts Medical School, Worcester, MA, USA/Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brazil | pt_BR |
Affilliation | Department of Cardiology, Philipps University, Marburg, Germany | pt_BR |
Affilliation | CNRS-UMR 8576, Unit of Structural and Functional Glycobiology. Univ Lille Nord de France, Lille, France/ Laboratory of Parasitology. Institute for Virology. Philipps University. Marburg, Germany | pt_BR |
Affilliation | Universite Francois Rabelais de Tours. UMR 1282 Infectiologie et Sante Publique. Tours, France/INRA, UMR 1282 Infectiologie et Sante Publique. Nouzilly, France/Laboratory of Parasitology. Institute for Virology. Philipps University. Marburg, Germany | pt_BR |
Subject | Trypanosoma cruzi | pt_BR |
Subject | Trypomastigote | pt_BR |
Subject | Amastigote | pt_BR |
Subject | Cardiomyocyte | pt_BR |
Subject | STAT3 Glycosylphosphatidylinositol | pt_BR |
Embargo date | 2020-01-01 | |