| Author | Oliveira, Edward José de | |
| Author | Saliba, Soraya Wilke | |
| Author | Saliba, Juliana Wilke | |
| Author | Rabello, Ana Lucia Teles | |
| Access date | 2018-11-26T17:09:30Z | |
| Available date | 2018-11-26T17:09:30Z | |
| Document date | 2013 | |
| Citation | OLIVEIRA, Edward José de et al. Validation of a direct agglutination test prototype kit for the diagnosis of visceral leishmaniasis. Trans R Soc Trop Med Hyg., v. 107, n. 4, p. 243-247, 2013 | pt_BR |
| ISSN | 0035-9203 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/30200 | |
| Language | eng | pt_BR |
| Publisher | Royal Society of Tropical Medicine and Hygiene | pt_BR |
| Rights | restricted access | pt_BR |
| Subject in Portuguese | Leishmaniose visceral | pt_BR |
| Subject in Portuguese | diagnostico sorologico | pt_BR |
| Subject in Portuguese | Kit de protótipo | pt_BR |
| Subject in Portuguese | DAT-LPC | pt_BR |
| Title | Validation of a direct agglutination test prototype kit for the diagnosis of visceral leishmaniasis | pt_BR |
| Type | Article | |
| DOI | 10.1093/trstmh/trt004 | |
| Abstract | Background: A freeze-dried antigen was developed with Leishmania (L.) infantum and used for the production of a prototype direct agglutination test kit for the laboratory diagnosis of visceral leishmaniasis (VL), called DAT-LPC. On this study the diagnosis validity of this prototype was performed.
Methods: To evaluate the sensitivity and specificity 103 samples from Brazilian patients with VL and 110 samples from patients with other parasitic infections, and healthy subjects were assayed with DAT-LPC and DAT-KIT (Royal Tropical Institute, Amsterdam, NL). Additionally, the results of 103 samples of VL patients based on two agglutination tests were transformed in Log10 and correlated.
Results: The DAT-LPC showed a sensitivity of 99.0%, specificity of 98.2% and diagnosis validity of 98.6%, which were similar to those found by the DAT-KIT (p.0.05). Moreover, there was positive correlation between the positive titers obtained by DAT-LPC and by DAT-KIT (Spearman correlation coefficient of 0.75 p ¼ 0.0001).
Conclusions: DAT-LPC showed thermal stability and diagnosis performance similar to those of the DAT-KIT. Our results suggest that DAT-LPC is a robust, simple, equipment-independent and efficient tool for the diagnosis of VL and should thus replace the IFAT as routine diagnostic test in the Brazilian public health system. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Laboratorio de Pesquisas Clinicas. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Laboratorio de Pesquisas Clinicas. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Laboratorio de Pesquisas Clinicas. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Laboratorio de Pesquisas Clinicas. Belo Horizonte, MG, Brazil | pt_BR |
| Subject | Visceral leishmaniasis | pt_BR |
| Subject | Serological diagnosis | pt_BR |
| Subject | Prototype kit | pt_BR |
| Subject | DAT-LPC | pt_BR |
| Embargo date | 2100-01-01 | |
