| Author | Tibiriçá, Eduardo | |
| Author | Matheus, Alessandra S. M. | |
| Author | Nunes, Bruno | |
| Author | Sperandei, Sandro | |
| Author | Gomes, Marilia B. | |
| Access date | 2018-11-26T19:19:43Z | |
| Available date | 2018-11-26T19:19:43Z | |
| Document date | 2011 | |
| Citation | TIBIRIÇÁ, Eduardo: et al. Repeatability of the evaluation of systemic microvascular endothelial function using laser doppler perfusion monitoring: clinical and statistical implications. Clinicas, v.66, n.4, p.599-605, Jan. 2011. | pt_BR |
| ISSN | 1807-5932 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/30210 | |
| Language | eng | pt_BR |
| Publisher | Universidade de São Paulo. Faculdade de Medicina | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Microcirculação da Pele | pt_BR |
| Subject in Portuguese | Estudos clínicos | pt_BR |
| Subject in Portuguese | Tamanho da amostra | pt_BR |
| Subject in Portuguese | Fluxometria por Doppler a Laser | pt_BR |
| Title | Repeatability of the evaluation of systemic microvascular endothelial function using laser doppler perfusion monitoring: clinical and statistical implications | pt_BR |
| Type | Article | |
| DOI | 10.1590/S1807-59322011000400013 | |
| Abstract | OBJECTIVE: An awareness of the repeatability of biological measures is required to properly design and calculate sample sizes for longitudinal interventional studies. We investigated the day-to-day repeatability of measures of systemic microvascular reactivity using laser Doppler perfusion monitoring.
METHODS: We performed laser Doppler perfusion monitoring in combination with skin iontophoresis using acetylcholine and sodium nitroprusside as well as post-occlusive reactive and thermal hyperemia twice within two weeks. The repeatability was assessed by calculating the within-subject standard deviations, limits of agreement, typical errors and intra-class correlation coefficients between days 1 and 2. The ratio of the within-subject standard deviation to the mean values obtained on days 1 and 2 (within-subject standard deviation/GM) was used to determine the condition with the best repeatability.
RESULTS: Twenty-four healthy subjects, aged 24.6¡3.8 years, were recruited. The area under the curve of the vasodilatory response to post-occlusive reactivity showed marked variability (within-subject standard deviation/GM =0.83), while the area under the curve for acetylcholine exhibited less variability (within-subject standard deviation/ GM =0.52) and was comparable to the responses to sodium nitroprusside and thermal treatment (within-subject standard deviations/GM of 0.67 and 0.56, respectively). The area under the blood flow/time curve for vasodilation during acetylcholine administration required the smallest sample sizes, the area under the blood flow/time curve during post-occlusive reactivity required the largest sample sizes, and the area under the blood flow/time curves of vasodilation induced by sodium nitroprusside and thermal treatment required intermediate sizes.
CONCLUSIONS: In view of the importance of random error related to the day-to-day repeatability of laser Doppler perfusion monitoring, we propose an original and robust statistical methodology for use in designing prospective clinical studies. | pt_BR |
| Affilliation | Universidade do Estado do Rio de Janeiro. Unidade de Diabetes. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Investigação Cardiovascular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Investigação Cardiovascular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade do Estado do Rio de Janeiro. Unidade de Diabetes. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Investigação Cardiovascular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Computacional e de Sistemas. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Investigação Cardiovascular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Skin Microcirculation | pt_BR |
| Subject | Laser Doppler Flowmetry | pt_BR |
| Subject | Clinical Studies | pt_BR |
| Subject | Sample Size | pt_BR |
| e-ISSN | 1980-5322 | |
