| Author | Melgaço, J. G. | |
| Author | Pinto, M. A. | |
| Author | Rocha, A. M. | |
| Author | Freire, M. | |
| Author | Gaspar, L. P. | |
| Author | Lima, S. M. B. | |
| Author | Cruz, O. G. | |
| Author | Vitral, C. L. | |
| Access date | 2018-11-27T15:02:41Z | |
| Available date | 2018-11-27T15:02:41Z | |
| Document date | 2011 | |
| Citation | MELGAÇO, J. G. et al. The Use of Dried Blood Spots for Assessing Antibody Response to Hepatitis A Virus After Natural Infection and Vaccination. Journal of Medical Virology, v.83, p.208–217, 2011. | pt_BR |
| ISSN | 0146-6615 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/30257 | |
| Language | eng | pt_BR |
| Publisher | Wiley | pt_BR |
| Rights | restricted access | |
| Subject in Portuguese | Hepatite A | pt_BR |
| Subject in Portuguese | coleta de sangue de papel de filtro | pt_BR |
| Subject in Portuguese | serovigilância | pt_BR |
| Subject in Portuguese | vacina contra hepatite A | pt_BR |
| Subject in Portuguese | Anticorpos Anti-Hepatite A | pt_BR |
| Title | The use of dried blood spots for assessing antibody response to hepatitis A virus after natural infection and vaccination | pt_BR |
| Type | Article | |
| DOI | 10.1002/jmv.21973 | |
| Abstract | During recent years, vaccination against hepatitis A has been implemented in several countries. It is expected that the increase in mass vaccination against hepatitis A will eventually result in a decreased prevalence of anti-HAV antibodies in the general population. For this reason, a suitable clinical sample for diagnosis of hepatitis A must be sufficiently sensitive to enable detection of lower antibodies titers. In this study, the feasibility of using dried blood spots (DBS) was assessed for the detection of anti-HAV antibodies after a natural infection and vaccination. Seventy-four DBS and paired plasma samples were obtained from a group of college students for a cross-sectional hepatitis A seroepidemiological study. Forty-six students seronegative for anti-HAV were selected randomly and immunized with an inactivated hepatitis A vaccine using an 0-6 month schedule. Seroconversion was monitored in paired plasma and DBS samples 6 months after the first dose followed by a period of 8 and 24 months after the second dose. A strong correlation between OD/CO rates of paired plasma and DBS samples for the detection of anti-HAV was observed. The sensitivity and specificity of the DBS compared with plasma for the detection of anti-HAV antibodies after natural infection was 100%. The sensitivity of DBS in samples collected 24 months after the second dose of hepatitis A vaccine was 95.4%. The results showed that DBS samples can be used for the detection of anti-HAV antibodies both after natural infection or vaccination. | pt_BR |
| Affilliation | Universidade Federal Fluminense. Instituto Biomédico. Departamento de Microbiologia e Parasitologia. Niterói, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Desenvolvimento Tecnológico em Virologia. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Universidade Federal Fluminense. Instituto Biomédico. Departamento de Microbiologia e Parasitologia. Niterói, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Laboratório de Tecnologia Virológica. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Laboratório de Tecnologia Virológica. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Laboratório de Tecnologia Virológica. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Programa de Computação Científica. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Universidade Federal Fluminense. Instituto Biomédico. Departamento de Microbiologia e Parasitologia. Niterói, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Desenvolvimento Tecnológico em Virologia. Rio de Janeiro, RJ. Brasil.. | pt_BR |
| Subject | hepatitis A | pt_BR |
| Subject | filter paper blood collection | pt_BR |
| Subject | anti-HAV | pt_BR |
| Subject | serosurveillance | pt_BR |
| Subject | hepatitis A vaccine | pt_BR |
| DeCS | Anticorpos Anti-Hepatite A | pt_BR |
| e-ISSN | 1096-9071 | |
| Embargo date | 2030-01-01 | |
| xmlui.metadata.dc.subject.ods | 03 Saúde e Bem-Estar | |
