| Author | Matrangolo, Fabiana da Silva Vieira | |
| Author | Liarte, Daniel Barbosa | |
| Author | Andrade, Laila de Carvalho | |
| Author | Melo, Melina Ferreira de | |
| Author | Andrade, Juvana Moreira | |
| Author | Ferreira, Rafael Fernandes | |
| Author | Santiago, André da Silva | |
| Author | Pirovani, Carlos Priminho | |
| Author | Pereira, Rosiane Aparecida da Silva | |
| Author | Murta, Silvane Maria Fonseca | |
| Access date | 2018-11-29T15:15:14Z | |
| Available date | 2018-11-29T15:15:14Z | |
| Document date | 2013 | |
| Citation | MATRANGOLO, Fabiana da Silva Vieira et al. Comparative proteomic analysis of antimony-resistant and -susceptible Leishmania braziliensis and Leishmania infantum chagasi lines. Molecular & Biochemical Parasitology, v. 190, p. 63– 75, 2013 | pt_BR |
| ISSN | 0166-6851 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/30326 | |
| Language | eng | pt_BR |
| Publisher | Elsevier | pt_BR |
| Rights | open access | pt_BR |
| Subject in Portuguese | Leishmania braziliensis | pt_BR |
| Subject in Portuguese | Leishmania infantum chagasi | pt_BR |
| Subject in Portuguese | resistencia a antimonio | pt_BR |
| Subject in Portuguese | proteoma | pt_BR |
| Subject in Portuguese | Eletroforese em Gel Bidimensional | pt_BR |
| Title | Comparative proteomic analysis of antimony-resistant and -susceptible Leishmania braziliensis and Leishmania infantum chagasi lines | pt_BR |
| Type | Article | pt_BR |
| DOI | 10.26719/2012.18.2.165 | |
| Abstract | The emergence of drug-resistant Leishmania species is a significant problem in several countries. A comparative proteomic analysis of antimony-susceptible and antimony-resistant Leishmania braziliensis (LbSbR) and Leishmania infantum chagasi (LcSbR) lines was carried out using two-dimensional gel electrophoresis (2-DE) followed by mass spectrometry (LC/MS/MS) for protein identification. Out of 132 protein spots exclusive or up-regulated submitted to MS, we identified 80 proteins that corresponded to 57 distinct proteins. Comparative analysis of data showed that most of the protein spots with differential abundance in both species are involved in antioxidant defense, general stress response, glucose and amino acid metabolism, and cytoskeleton organization. Five proteins were commonly more abundant in both SbIII-resistant Leishmania lines: tryparedoxin peroxidase, alpha-tubulin, HSP70, HSP83, and HSP60. Analysis of the protein abundance by Western blotting assays confirmed our proteomic data.
These assays revealed that cyclophilin-A is less expressed in both LbSbR and LcSbR lines. On the other hand, the expression of pteridine reductase is higher in the LbSbR line, whereas tryparedoxin peroxidase is overexpressed in both LbSbR and LcSbR lines. Together, these results show that the mechanism of antimony-resistance in Leishmania spp. is complex and multifactorial. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Parasitologia Celular e Molecular. Belo Horizonte, MG, Brazil/Universidade Estadual de Montes Claros. Laboratório de Epidemiologia e Biocontrole de Microrganismos. Montes Claros, Brazil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Parasitologia Celular e Molecular. Belo Horizonte, MG, Brazil/ Universidade Federal do Piauí. Piauí, Brazil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Parasitologia Celular e Molecular. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Parasitologia Celular e Molecular. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Parasitologia Celular e Molecular. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Parasitologia Celular e Molecular. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Universidade Estadual de Santa Cruz. Laboratório de Proteômica. Ilheús, BA, Brazil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Parasitologia Celular e Molecular. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Parasitologia Celular e Molecular. Belo Horizonte, MG, Brazil | pt_BR |
| Subject | Leishmania braziliensis | pt_BR |
| Subject | Leishmania infantum chagasi | pt_BR |
| Subject | Antimony resistance | pt_BR |
| Subject | Proteome | pt_BR |
| Subject | Electrophoresis, Gel, Two-Dimensional | pt_BR |
| Embargo date | 2020-01-01 | |
