| Author | Coutinho, Joana O P A | |
| Author | Silva, Mariana Pereira dos Santos | |
| Author | Moraes, P.M. | |
| Author | Monteiro, Andrea de Souza | |
| Author | Barcelos, J.C.C. | |
| Author | Siqueira Filho, Ezequias Pessoa de | |
| Author | Santos, Vera Lúcia dos | |
| Access date | 2018-12-05T16:28:12Z | |
| Available date | 2018-12-05T16:28:12Z | |
| Document date | 2013 | |
| Citation | COUTINHO, Joana O P A et al. Demulsifying properties of extracellular products and cells of Pseudomonas aeruginosa MSJ isolated from petroleum-contaminated soil. Bioresour Technol., v. 128, p. 646-654, 2013 | pt_BR |
| ISSN | 0960-8524 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/30403 | |
| Language | eng | pt_BR |
| Publisher | Elsevier Science Pub. Co | pt_BR |
| Rights | open access | pt_BR |
| Subject in Portuguese | Pseudomonas aeruginosa | pt_BR |
| Subject in Portuguese | Demulsificação | pt_BR |
| Subject in Portuguese | Emulsões de água em óleo | pt_BR |
| Title | Demulsifying properties of extracellular products and cells of Pseudomonas aeruginosa MSJ isolated from petroleum-contaminated soil | pt_BR |
| Type | Article | pt_BR |
| DOI | 10.1016/j.biortech.2012.09.137 | |
| Abstract | A strain of Pseudomonas aeruginosa isolated from a site contaminated with refined oil products exhibited demulsification capabilities against Tween 80-Span 80 stabilized oil-in-water (O/W), Tween 80-stabilized water-in-oil (W/O) model emulsions (kerosene-water), and an industrial emulsion (Daido Dairoll PA-5A). GC-MS analysis confirmed the presence of fatty acids and carbohydrates in the extracellular biodemulsifier. The demulsifying activity of cells and culture supernatants was favored by growth in media containing 1% diesel oil. There was a correlation between culture age, de-emulsification and cellular hydrophobicity, and highest activities were observed for cells and supernatants from 96-h cultures. Activity increased with addition of up to 60 mg cells or 300 μL supernatant to emulsions. The activity was relatively stable at 20-40 °C and to freezing, but was reduced by 69% by washing the cells with chloroform-methanol-water. This demulsifier has potential for application in biotreatment of emulsified oily wastewaters to promote recovery and/or degradation of oil. | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG, Brazil/Universidade Vale do Rio Doce. Faculdade de Ciências da Saúde. Laboratório de Pesquisa em Microbiologia. MG, Brazil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Química de Produtos Naturais. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG, Brazil | pt_BR |
| Subject | Pseudomonas aeruginosa | pt_BR |
| Subject | Demulsification | pt_BR |
| Subject | Water-in-oil emulsions | pt_BR |
| Embargo date | 2020-01-01 | |
