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dc.contributor.authorVillabona-Arenas, Christian Julian
dc.contributor.authorMondini, Adriano
dc.contributor.authorBosch, Irene
dc.contributor.authorSchimitt, Diane
dc.contributor.authorSilva, Carlos Eduardo Calzavara
dc.contributor.authorZanotto, Paolo Marinho de Andrade
dc.contributor.authorNogueira, Maurício Lacerda
dc.date.accessioned2018-12-05T17:04:25Z
dc.date.available2018-12-05T17:04:25Z
dc.date.issued2013
dc.identifier.citationVILLABONA-ARENAS, Christian Julian et al. Dengue virus type 3 adaptive changes during epidemics in São Jose de Rio Preto, Brazil, 2006-2007. PLoS One., v. 8, n. 5, e63496, 2013
dc.identifier.issn1932-6203
dc.identifier.urihttps://www.arca.fiocruz.br/handle/icict/30405
dc.language.isoeng
dc.publisherPublic Library of Science
dc.rightsopen access
dc.subject.otherDengue virus
dc.titleDengue virus type 3 adaptive changes during epidemics in São Jose de Rio Preto, Brazil, 2006-2007
dc.typeArticle
dc.identifier.doi10.1371/journal.pone.0063496
dc.description.abstractenGlobal dengue virus spread in tropical and sub-tropical regions has become a major international public health concern. It is evident that DENV genetic diversity plays a significant role in the immunopathology of the disease and that the identification of polymorphisms associated with adaptive responses is important for vaccine development. The investigation of naturally occurring genomic variants may play an important role in the comprehension of different adaptive strategies used by these mutants to evade the human immune system. In order to elucidate this role we sequenced the complete polyprotein-coding region of thirty-three DENV-3 isolates to characterize variants circulating under high endemicity in the city of São José de Rio Preto, Brazil, during the onset of the 2006-07 epidemic. By inferring the evolutionary history on a local-scale and estimating rates of synonymous (dS) and nonsynonimous (dN) substitutions, we have documented at least two different introductions of DENV-3 into the city and detected 10 polymorphic codon sites under significant positive selection (dN/dS > 1) and 8 under significant purifying selection (dN/dS < 1). We found several polymorphic amino acid coding sites in the envelope (15), NS1 (17), NS2A (11), and NS5 (24) genes, which suggests that these genes may be experiencing relatively recent adaptive changes. Furthermore, some polymorphisms correlated with changes in the immunogenicity of several epitopes. Our study highlights the existence of significant and informative DENV variability at the spatio-temporal scale of an urban outbreak.
dc.creator.affilliationUniversidade de São Paulo. Instituto de Ciências Biomédicas. Departamento de Microbiologia. Laboratório de Evolução Molecular e Bioinformática. São Paulo, Brazil
dc.creator.affilliationUniversidade Estadual Paulista “Júlio de Mesquita Filho”. Faculdade de Ciências Farmacêuticas. Departamento de Ciências Biológicas. Laboratório de Saúde Pública. Araraquara, SP, Brazil
dc.creator.affilliationDivision of Heath Science and Technology. Massachusetts Institute of Technology. Cambridge, MA, United States of America
dc.creator.affilliationDepartment of Infectious Disease and Global Health. Cummings School of Veterinary Medicine. Tufts University. North Grafton, MA, United States of America
dc.creator.affilliationFundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Laboratório de Imunologia Celular e Molecular. Belo Horizonte, MG, Brazil
dc.creator.affilliationUniversidade de São Paulo. Instituto de Ciências Biomédicas. Departamento de Microbiologia. Laboratório de Evolução Molecular e Bioinformática. São Paulo, SP, Brazil
dc.creator.affilliationFaculdade de Medicina de São José do Rio Preto. Laboratório de Pesquisas em Virologia. São José do Rio Preto, SP, Brazil
dc.subject.endengue virus
Appears in Collections:MG - IRR - Artigos de Periódicos

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