| Author | Genta, Fernando A. | |
| Author | Souza, R. S. | |
| Author | Garcia, E. S. | |
| Author | Azambuja, P. | |
| Access date | 2018-12-11T17:01:43Z | |
| Available date | 2018-12-11T17:01:43Z | |
| Document date | 2010 | |
| Citation | GENTA, Fernando A.; et al. Phenol oxidases from Rhodnius prolixus: Temporal and tissue expression pattern and regulation by ecdysone. Journal of Insect Physiology, v. 56, p.1253–1259, 2010. | pt_BR |
| ISSN | 0022-1910 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/30522 | |
| Language | eng | pt_BR |
| Publisher | Elsevier | pt_BR |
| Rights | restricted access | pt_BR |
| Subject in Portuguese | Rhodnius prolixus | pt_BR |
| Subject in Portuguese | Corpo Adiposo | pt_BR |
| Subject in Portuguese | Intestino | pt_BR |
| Title | Phenol oxidases from Rhodnius prolixus: temporal and tissue expression pattern and regulation by ecdysone | pt_BR |
| Type | Article | pt_BR |
| DOI | 10.1016/j.jinsphys.2010.03.027 | |
| Abstract | Rhodnius prolixus 5th instar nymphs have significant PO enzymatic activity in the anterior midgut, fat body and hemolymph. The tissue with the major amount of PO activity is the anterior midgut while those with higher specific activities are the fat body and hemolymph. In this work the temporal pattern of PO enzymatic activity in different tissues was investigated. In fat body, PO peaks occur at 7, 12 and 16 days after a blood meal. In hemolymph, PO diminishes until day 7, and then recovers by day 14. In the anterior midgut tissue, PO peaks on day 9 and just before ecdysis; a similar pattern was observed in the anterior midgut contents. Some of these activities are dependent on the release of ecdysone, as feeding blood meal containing azadirachtin suppresses them and ecdysone treatment counteracts this effect. These results suggest that during the development of the 5th instar, the insect has natural regulating cycles of basal PO expression and activation, which could be related to the occurrence of natural infections. The differences in temporal patterns of activity and the effects of azadirachtin and ecdysone in each organ suggest that, at least in R. prolixus, different tissues are expressing different PO genes. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ. Brasil / Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular. Rio de Janeiro, RJ, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ. Brasil / Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular. Rio de Janeiro, RJ, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ. Brasil / Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular. Rio de Janeiro, RJ, Brasil | pt_BR |
| Subject | Phenoloxidase | pt_BR |
| Subject | Rhodnius prolixus | pt_BR |
| Subject | Fat body | pt_BR |
| Subject | Midgut | pt_BR |
| Subject | Hemolymph | pt_BR |
| Subject | Ecdysone | pt_BR |
| Subject | Azadirachtin | pt_BR |
| DeCS | Monofenol Mono-Oxigenase | pt_BR |
| DeCS | Ecdisona | pt_BR |
| DeCS | Hemolinfa | pt_BR |
| e-ISSN | 1879-1611 | |
| Embargo date | 2030-01-01 | |
