Author | Martins, Gabriele Louise Soares | |
Author | Paredes, Bruno Diaz | |
Author | Azevedo, Carine Machado | |
Author | Sampaio, Gabriela Louise de Almeida | |
Author | Nonaka, Carolina Kymie Vasques | |
Author | Cavalcante, Bruno Raphael Ribeiro | |
Author | Silva, Katia Nunes da | |
Author | Pereira, Ciro Silveira e | |
Author | Soares, Milena Botelho Pereira | |
Author | Santos, Ricardo Ribeiro dos | |
Author | Souza, Bruno Solano de Freitas | |
Access date | 2018-12-13T13:38:50Z | |
Available date | 2018-12-13T13:38:50Z | |
Document date | 2018 | |
Citation | MARTINS, G. L. S. et al. Generation of integration-free iPS cell lines from three sickle cell disease patients from the state of Bahia, Brazil. Stem Cell Research, v. 33, p. 10-14, 2018. | pt_BR |
ISSN | 1873-5061 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/30572 | |
Sponsorship | CNPq, CAPES, FAPESB,
Ministry of Health, Brazil National Induced Pluripotent Stem Cell
Biobank, Brazil and the National Institute of Science and Technology
for Regenerative Medicine, Brazil. | pt_BR |
Language | eng | pt_BR |
Publisher | Elsevier | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Doença falciforme | pt_BR |
Subject in Portuguese | Celulas tronco | pt_BR |
Subject in Portuguese | Diversidade genética | pt_BR |
Subject in Portuguese | Humanos | pt_BR |
Subject in Portuguese | Bahia | pt_BR |
Title | Generation of integration-free iPS cell lines from three sickle cell disease patients from the state of Bahia, Brazil | pt_BR |
Type | Article | pt_BR |
DOI | 10.1016/j.scr.2018.09.011 | |
Abstract | Sickle cell disease (SCD) is one of the most prevalent and severe monogenetic disorders, affecting several million people around the world. Clinical manifestations
and complications of the disease include sickle cell pain crisis, silent cerebral infarct, stroke, nephropathy and early death. In this study, we generated induced
pluripotent stem cell (iPSC) lines from three homozygous SCD patients from the state of Bahia, Brazil, where SCD is highly prevalent. Peripheral blood mononuclear
cells were collected and erythroblasts were expanded for cell reprogramming with the use of non-integrative episomal vectors. The generated iPSC lines expressed
high levels of pluripotency markers, presented a normal karyotype and were able to differentiate into the three germ layers in embryoid body spontaneous differentiation
assays. Moreover, the expression of the episomal vectors was lost in all iPSC lines after 15 passages. These iPSC lines may help increasing the knowledge
about SCD pathogenesis and can be a useful tool for drug testing and gene editing studies. | pt_BR |
Affilliation | São Rafael Hospital. Center for Biotechnology and Cell Therapy. Salvador, BA, Brazil / Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
Affilliation | São Rafael Hospital. Center for Biotechnology and Cell Therapy. Salvador, BA, Brazil / National Institute of Science and Technology for Regenerative Medicine. Rio de Janeiro, RJ, Brazil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
Affilliation | São Rafael Hospital. Center for Biotechnology and Cell Therapy. Salvador, BA, Brazil / Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
Affilliation | São Rafael Hospital. Center for Biotechnology and Cell Therapy. Salvador, BA, Brazil / Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
Affilliation | São Rafael Hospital. Center for Biotechnology and Cell Therapy. Salvador, BA, Brazil | pt_BR |
Affilliation | São Rafael Hospital. Center for Biotechnology and Cell Therapy. Salvador, BA, Brazil / National Institute of Science and Technology for Regenerative Medicine. Rio de Janeiro, RJ, Brazil. | pt_BR |
Affilliation | São Rafael Hospital. Center for Biotechnology and Cell Therapy. Salvador, BA, Brazil | pt_BR |
Affilliation | São Rafael Hospital. Center for Biotechnology and Cell Therapy. Salvador, BA, Brazil / Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / National Institute of Science and Technology for Regenerative Medicine. Rio de Janeiro, RJ, Brazil. | pt_BR |
Affilliation | São Rafael Hospital. Center for Biotechnology and Cell Therapy. Salvador, BA, Brazil / Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / National Institute of Science and Technology for Regenerative Medicine. Rio de Janeiro, RJ, Brazil. | pt_BR |
Affilliation | São Rafael Hospital. Center for Biotechnology and Cell Therapy. Salvador, BA, Brazil / Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / National Institute of Science and Technology for Regenerative Medicine. Rio de Janeiro, RJ, Brazil. | pt_BR |
Subject | Sickle cell disease | pt_BR |
Subject | Stem cells | pt_BR |
Subject | Genetical diversity | pt_BR |
Subject | Humans | pt_BR |
Subject | Bahia | pt_BR |