| Author | Silva, Daniela Nascimento | |
| Author | Souza, Bruno Solano de Freitas | |
| Author | Azevedo, Carine Machado | |
| Author | Vasconcelos, Juliana Fraga | |
| Author | Jesus, Paloma Gomes de | |
| Author | Feitoza, Malena Sebastiana Barreto | |
| Author | Meira, Cassio Santana | |
| Author | Carvalho, Gisele B | |
| Author | Cavalcante, Bruno Raphael Ribeiro | |
| Author | Santos, Ricardo Ribeiro dos | |
| Author | Soares, Milena Botelho Pereira | |
| Access date | 2018-12-14T13:17:36Z | |
| Available date | 2018-12-14T13:17:36Z | |
| Document date | 2018 | |
| Citation | SILVA, D. N. et al. IGF-1-Overexpressing Mesenchymal Stem/Stromal Cells Promote Immunomodulatory and Proregenerative Effects in Chronic Experimental Chagas Disease. Stem Cells International, ID 9108681, 2018. | pt_BR |
| ISSN | 1687-966X | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/30596 | |
| Sponsorship | FINEP, CNPq, and FAPESB for research funding. | pt_BR |
| Language | eng | pt_BR |
| Publisher | Hindawi Publishing Corporation | pt_BR |
| Rights | open access | pt_BR |
| Subject in Portuguese | Células tronco | pt_BR |
| Subject in Portuguese | Células Mesenquimais | pt_BR |
| Subject in Portuguese | Doença de Chagas | pt_BR |
| Subject in Portuguese | Células estaminais | pt_BR |
| Subject in Portuguese | Sistema cardiovascular | pt_BR |
| Subject in Portuguese | Transplante de células-tronco | pt_BR |
| Subject in Portuguese | Musculo cardíaco | pt_BR |
| Subject in Portuguese | Musculo esquelético | pt_BR |
| Title | IGF-1-Overexpressing Mesenchymal Stem/Stromal Cells Promote Immunomodulatory and Proregenerative Effects in Chronic Experimental Chagas Disease | pt_BR |
| Type | Article | pt_BR |
| DOI | 10.1155/2018/9108681 | |
| Abstract | Mesenchymal stem/stromal cells (MSCs) have been investigated for the treatment of diseases that affect the cardiovascular system, including Chagas disease. MSCs are able to promote their beneficial actions through the secretion of proregenerative and immunomodulatory factors, including insulin-like growth factor-1 (IGF-1), which has proregenerative actions in the heart and skeletal muscle. Here, we evaluated the therapeutic potential of IGF-1-overexpressing MSCs (MSC_IGF-1) in a mouse model of chronic Chagas disease. C57BL/6 mice were infected with Colombian strain Trypanosoma cruzi and treated with MSCs, MSC_IGF-1, or vehicle (saline) six months after infection. RT-qPCR analysis confirmed the presence of transplanted cells in both the heart and skeletal muscle tissues. Transplantation of either MSCs or MSC_IGF-1 reduced the number of inflammatory cells in the heart when compared to saline controls. Moreover, treatment with MSCs or MSC_IGF-1 significantly reduced TNF-α, but only MSC treatment reduced IFN-γ production compared to the saline group. Skeletal muscle sections of both MSC- and MSC_IGF-1-treated mice showed a reduction in fibrosis compared to saline controls. Importantly, the myofiber area was reduced in T. cruzi-infected mice, and this was recovered after treatment with MSC_IGF-1. Gene expression analysis in the skeletal muscle showed a higher expression of pro- and anti-inflammatory molecules in MSC_IGF-1-treated mice compared to MSCs alone, which significantly reduced the expression of TNF-α and IL-1β. In conclusion, our results indicate the therapeutic potential of MSC_IGF-1, with combined immunomodulatory and proregenerative actions to the cardiac and skeletal muscles. | pt_BR |
| Affilliation | Hospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Hospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / National Institute of Science and Technology for Regenerative Medicine. Rio de Janeiro, RJ, Brazil. | pt_BR |
| Affilliation | Hospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Hospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Hospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, Brasil | pt_BR |
| Affilliation | Hospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, Brasil | pt_BR |
| Affilliation | Hospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, Brasil | pt_BR |
| Affilliation | Hospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, Brasil | pt_BR |
| Affilliation | Hospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, Brasil | pt_BR |
| Affilliation | Hospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, Brasil / National Institute of Science and Technology for Regenerative Medicine. Rio de Janeiro, RJ, Brazil. | pt_BR |
| Affilliation | Hospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / National Institute of Science and Technology for Regenerative Medicine. Rio de Janeiro, RJ, Brazil. | pt_BR |
| Subject | Stem Cells | pt_BR |
| Subject | Mesenchymal Cells | pt_BR |
| Subject | Chagas disease | pt_BR |
| Subject | Stromal cells | pt_BR |
| Subject | Cardiovascular System | pt_BR |
| Subject | Stem Cell Transplantation | pt_BR |
| Subject | Myocardium | pt_BR |
| Subject | Muscle, Skeletal | pt_BR |
| xmlui.metadata.dc.subject.ods | 03 Saúde e Bem-Estar | |
