Mostrar el registro sencillo del ítem

AutorFukutani, Kiyoshi Ferreira
AutorCarvalho, Cristiana Maria Costa Nascimento de
AutorBouzas, Maiara L.
AutorOliveira, Juliana R.
AutorBarral, Aldina Maria Prado
AutorDierckx, Tim
Autorunha, Antonio Ricardo Khouri
AutorNakaya, Helder Imoto Takashi
AutorAndrade, Bruno de Bezerril
AutorVan Weyenbergh, Johan
AutorOliveira, Camila Indiani de
Fecha de acceso2018-12-18T14:08:09Z
Fecha de disponibilización2018-12-18T14:08:09Z
Fecha de publicación2018
ReferenciaFUKUTANI, Kiyoshi Ferreira et al. In situ immune signatures and microbial load at the nasopharyngeal interface in children with acute respiratory infection. Frontiers in Microbiology, v. 9, p. 1-13, 2018.pt_BR
ISSN1664-302Xpt_BR
URIhttps://www.arca.fiocruz.br/handle/icict/30656
PromociónFundação de Amparo à Pesquisa do Estado da Bahia (FAPESB; Grant PNX0019/2009 to AB) and Belgian FWO (Grant G0D6817N). KF was supported by a fellowship from Science without Borders (PVE Anne- Mieke Vandamme). TD was supported by the Institute for the Promotion of Innovation through Science and Technology Flanders (IWT-Vlaanderen), project 141614. CN-C, AB, and CO were senior investigators from Conselho Nacional de Pesquisa Científica (CNPq). HN was supported by FAPESP (Grant Nos. 2012/19278-6 and 2017/50137-3). BA was supported by the National Institutes of Health (U01AI115940).pt_BR
Idiomaengpt_BR
EditorFrontiers Mediapt_BR
Derechos de autoropen accesspt_BR
Palabras clave en PortuguésAnálise de redespt_BR
Palabras clave en PortuguésIRApt_BR
Palabras clave en PortuguésResposta imunept_BR
Palabras clave en PortuguésnContadorpt_BR
Palabras clave en PortuguésIinterferonpt_BR
Palabras clave en PortuguésCarga viralpt_BR
Palabras clave en Portuguésimunidade inatapt_BR
Palabras clave en PortuguésImunidade adaptativapt_BR
TítuloIn situ Immune Signatures and Microbial Load at the Nasopharyngeal Interface in Children With Acute Respiratory Infectionpt_BR
Tipo del documentoArticlept_BR
DOI10.3389/fmicb.2018.02475
Resumen en InglésAcute respiratory infection (ARI) is the most frequent cause for hospitalization in infants and young children. Using multiplexed nCounter technology to digitally quantify 600 human mRNAs in parallel with 14 virus- and 5 bacterium-specific RNAs, we characterized viral and bacterial presence in nasopharyngeal aspirates (NPA) of 58 children with ARI and determined the corresponding in situ immune profiles. NPA contained different groups of organisms and these were classified into bacterial (n = 27), viral (n = 5), codetection [containing both viral and bacterial transcripts (n = 21), or indeterminate intermediate where microbial load is below threshold (n = 5)]. We then identified differentially expressed immune transcripts (DEITs) comparing NPAs from symptomatic children vs. healthy controls, and comparing children presenting NPAs with detectable microbial load vs. indeterminate. We observed a strong innate immune response in NPAs, due to the presence of evolutionarily conserved type I Interferon (IFN)-stimulated genes (ISG), which was correlated with total bacterial and/or viral load. In comparison with indeterminate NPAs, adaptive immunity transcripts discriminated among viral, bacterial, and codetected microbial profiles. In viral NPAs, B cell transcripts were significantly enriched among DEITs, while only type III IFN was correlated with viral load. In bacterial NPAs, myeloid cells and coinhibitory transcripts were enriched and significantly correlated with bacterial load. In conclusion, digital nCounter transcriptomics provide a microbial and immunological in situ "snapshot" of the nasopharyngeal interface in children with ARI. This enabled discrimination among viral, bacterial, codetection, and indeterminate transcripts in the samples using non-invasive sampling.pt_BR
AfiliaciónFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.pt_BR
AfiliaciónFederal University of Bahia. School of Medicine. Salvador, BA, Brazil / Federal University of Bahia. School of Medicine. Department of Pediatrics. Salvador, BA, Brazil.pt_BR
AfiliaciónFederal University of Bahia. School of Medicine. Department of Pediatrics. Salvador, BA, Brazil.pt_BR
AfiliaciónFederal University of Bahia. School of Medicine. Department of Pediatrics. Salvador, BA, Brazil.pt_BR
AfiliaciónFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Federal University of Bahia. School of Medicine. Department of Pediatrics. Salvador, BA, Brazil.pt_BR
AfiliaciónRega Institute for Medical Research. Department of Microbiology and Immunology. KU Leuven, Leuven, Belgium.pt_BR
AfiliaciónFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Federal University of Bahia. School of Medicine. Department of Pediatrics. Salvador, BA, Brazil.pt_BR
AfiliaciónUniversity of São Paulo. School of Pharmaceutical Sciences. Department of Clinical and Toxicological Analyses. São Paulo, SP, Brazil.pt_BR
AfiliaciónFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Fundação José Silveira. Multinational Organization Network Sponsoring Translational and Epidemiological Research Initiative. Salvador, BA, Brasil.pt_BR
AfiliaciónRega Institute for Medical Research. Department of Microbiology and Immunology. KU Leuven, Leuven, Belgium.pt_BR
AfiliaciónFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Federal University of Bahia. School of Medicine. Department of Pediatrics. Salvador, BA, Brazil.pt_BR
Palavras clave en InglêsNetwork analysispt_BR
Palavras clave en InglêsARIpt_BR
Palavras clave en InglêsImmune responsept_BR
Palavras clave en InglêsnCounterpt_BR
Palavras clave en InglêsInterferonpt_BR
Palavras clave en InglêsViral loadpt_BR
Palavras clave en InglêsInnate immunitypt_BR
Palavras clave en InglêsAdaptive immunitypt_BR


Ficheros en el ítem

application/pdf
Nombre:
Fukutani K. In situ immune ...
Tamaño:
3.943Mb
Formato:
application/
Ver/Abrir

Este ítem aparece en la(s) siguiente(s) colección(ones)

Mostrar el registro sencillo del ítem