| Author | Ferreira, Alex Guerra | |
| Author | Leão, Robson Souza | |
| Author | Carvalho-Assef, Ana Paula D 'A. | |
| Author | Folescu, Tania Wrobel | |
| Author | Barth, Afonso Luís | |
| Author | Marques, Elizabeth Andrade | |
| Access date | 2018-12-18T16:16:55Z | |
| Available date | 2018-12-18T16:16:55Z | |
| Document date | 2010 | |
| Citation | FERREIRA, Alex Guerra; et al. Influence of biofilm formation in the susceptibility of Pseudomonas aeruginosa from Brazilian patients cystic fibrosis. APMIS, v.118, p.606-612, 2010. | pt_BR |
| ISSN | 0903-4641 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/30669 | |
| Language | eng | pt_BR |
| Publisher | Wiley | pt_BR |
| Rights | restricted access | |
| Subject in Portuguese | Pseudomonas aeruginosa | pt_BR |
| Subject in Portuguese | Fibrose sística | pt_BR |
| Subject in Portuguese | biofilme | pt_BR |
| Subject in Portuguese | resistência | pt_BR |
| Title | Influence of biofilm formation in the susceptibility of Pseudomonas aeruginosa from Brazilian patients with cystic fibrosis | pt_BR |
| Type | Article | |
| DOI | 10.1111/j.1600-0463.2010.02636.x | |
| Abstract | Biofilms play a key role in the occurrence of lung infections by Pseudomonas aeruginosa in patients with cystic fibrosis (CF). In this study, we examined 40 isolates of P. aeruginosa from CF patients according to their capacity to form biofilm. We also compared their in vitro response to antimicrobials according to different modes of growth (planktonic vs biofilm) and performed molecular typing. All isolates proved capable of forming biofilm. However, there was no difference in biofilm development according to the mucoid and nonmucoid phenotypes and among isolates obtained at different periods of the chronic infection. All isolates tested for antimicrobial susceptibility in the biofilm state (BIC) were consistently more resistant to antibiotics than the same isolate tested in the planktonic state. The molecular typing indicates a considerable clonal diversity among isolates. We identified five patients harboring the same strain over different periods. These strains, however, displayed different levels of biofilm formation and BIC values for antibiotics tested. The results of the present study demonstrate that there is a marked difference in the susceptibility profile according to the mode of growth of CF P. aeruginosa, as cells tested in the biofilm state proved consistently more resistant to antibiotics. | pt_BR |
| Affilliation | Universidade do Estado do Rio de Janeiro. Faculdade de Ciências Médicas. Departamento de Microbiologia e Imunologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade do Estado do Rio de Janeiro. Faculdade de Ciências Médicas. Departamento de Microbiologia e Imunologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Infecção Hospitalar. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Fernandes Figueira. Serviço de Pneumologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio Grande do Sul. Unidade de Microbiologia e Biologia Molecular. RS, Brasil. | pt_BR |
| Affilliation | Universidade do Estado do Rio de Janeiro. Faculdade de Ciências Médicas. Departamento de Microbiologia e Imunologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Pseudomonas aeruginosa | pt_BR |
| Subject | cystic fibrosis | pt_BR |
| Subject | biofilm | pt_BR |
| Subject | resistance | pt_BR |
| e-ISSN | 1600-0463 | |
| Embargo date | 2030-01-01 | |
