| Author | Miranda, Iranaia Assunção | |
| Author | Amaral, Flavio A. | |
| Author | Bozza, Fernando A. | |
| Author | Fagundes, Caio T. | |
| Author | Souza, Lirlandia P. | |
| Author | Souza, Danielle G. | |
| Author | Pacheco, Patrícia | |
| Author | Lima, Giselle Barbosa | |
| Author | Gomes, Rachel N. | |
| Author | Bozza, Patrícia T. | |
| Author | Poian, Andrea T. da | |
| Author | Teixeira, Mauro M. | |
| Author | Bozza, Marcelo T. | |
| Access date | 2018-12-18T16:55:46Z | |
| Available date | 2018-12-18T16:55:46Z | |
| Document date | 2010 | |
| Citation | MIRANDA, Iranaia Assunção et al. Contribution of macrophage migration inhibitory factor to the pathogenesis of dengue virus infection. FASEB Journal, v. 24, p. 218-228, Jan. 2010. | pt_BR |
| ISSN | 0892-6638 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/30675 | |
| Language | eng | pt_BR |
| Publisher | Federation of American Society of Experimental Biology | pt_BR |
| Rights | restricted access | |
| Subject in Portuguese | Sepse | pt_BR |
| Subject in Portuguese | Febre hemorrágia | pt_BR |
| Subject in Portuguese | Inflamação | pt_BR |
| Subject in Portuguese | Gotículas lipídicas | pt_BR |
| Subject in Portuguese | Citocinas | pt_BR |
| Subject in Portuguese | Dengue | pt_BR |
| Title | Contribution of macrophage migration inhibitory factor to the pathogenesis of dengue virus infection | pt_BR |
| Type | Article | |
| DOI | 10.1096/fj.09-139469 | pt_BR |
| Abstract | Dengue fever is an emerging viral disease transmitted by arthropods to humans in tropical countries. Dengue hemorrhagic fever (DHF) is escalating
in frequency and mortality rates. Here we studied the involvement of macrophage migration inhibitory factor (MIF) in dengue virus (DENV) infection and its pathogenesis. Patients with DHF had elevated plasma concentrations of MIF. Both leukocytes from these patients and macrophages from healthy donors infected in vitro with DENV showed a substantial amount of MIF within lipid droplets. The secretion of MIF by
macrophages and hepatocytes required a productive infection and occurred without an increase in gene transcription or cell death, thus indicating active secretion from preformed stocks. In vivo infection of wildtype and mif-deficient (Mif /) mice demonstrated a role of MIF in dengue pathogenesis. Clinical disease was less severe in Mif / mice, and they exhibited a
significant delay in lethality, lower viremia, and lower viral load in the spleen than wild-type mice. This reduction in all parameters of severity on DENV infection in Mif / mice correlated with reduced proinflammatory cytokine concentrations. These results demonstrated the contribution of MIF to the pathogenesis of dengue and pointed to a possible beneficial role of
neutralizing MIF as an adjunctive therapeutic approach to treat the severe forms of the disease. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica. Programa de Biologia Estrutural. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Microbiologia. Departamento de Imunologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Departamento de Microbiologia. MG, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Unidade de Cuidados Intensivos. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Departamento de Microbiologia. MG, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Minas Gerais, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Departamento de Microbiologia. MG, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica. Programa de Biologia Estrutural. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Minas Gerais, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Microbiologia. Departamento de Imunologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | MIF | pt_BR |
| Subject | Cytokine | pt_BR |
| Subject | Lipid droplets | pt_BR |
| Subject | Inflammation | pt_BR |
| Subject | Hemorrhagic fever | pt_BR |
| Subject | Sepsis | pt_BR |
| Subject | Dengue | pt_BR |
| e-ISSN | 1530-6860 | |
| Embargo date | 2030-01-01 | |
