Author | Almeida, Marcos Abreu | |
Author | Damasceno, Lisandra Serra | |
Author | Pizzini, Cláudia Vera | |
Author | Muniz, Mauro de Medeiros | |
Author | Almeida‐Paes, Rodrigo | |
Author | Zancopé‐Oliveira, Rosely Maria | |
Access date | 2018-12-27T12:47:29Z | |
Available date | 2018-12-27T12:47:29Z | |
Document date | 2018 | |
Citation | ALMEIDA, Marcos Abreu et al. Role of western blot assay for the diagnosis of histoplasmosis in AIDS patients from a National Institute of Infectious Diseases in Rio de Janeiro, Brazil. Mycoses, p. 1-22, Dec. 18, 2018. | pt_BR |
ISSN | 0933-7407 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/30788 | |
Language | eng | pt_BR |
Publisher | Wiley | pt_BR |
Rights | open access | pt_BR |
Title | Role of western blot assay for the diagnosis of histoplasmosis in AIDS patients from a National Institute of Infectious Diseases in Rio de Janeiro, Brazil | pt_BR |
Type | Preprint | pt_BR |
DOI | 10.1111/myc.12877 | |
Abstract | BACKGROUND: Histoplasmosis is a frequent fungal infection in HIV/AIDS patients, with high morbimortality rates when diagnosis and treatment are delayed. Antibody detection, which is faster than the gold standard culture test, hasten the laboratory investigation. OBJECTIVES: To evaluate the role of WB for antibody detection in the diagnosis of histoplasmosis among HIV/AIDS patients. PATIENTS AND METHODS: Fifty patients with proven or probable histoplasmosis were included. Clinical, epidemiological and laboratory data were described in the same population after a review of their medical records. WB was performed using deglycosylated histoplasmin. RESULTS: 82% of patients were adult males and the mean age was 39.3 years. CD4+ T lymphocyte count less than 150 cells/mm3 was observed in 62% patients. Antibodies against Histoplasma capsulatum M antigen were detected in 62% of patients, and against both M and H antigens in 28% of individuals. Sera from 10% of patients were nonreactive. Histoplasmosis was the first opportunistic infection in 38% of the cases. Disseminated and pulmonary histoplasmosis occurred in 84% and 16% of patients, respectively. The overall mortality was 16%. CONCLUSION: WB could be useful for the histoplasmosis diagnosis in HIV/AIDS patients because of its easefulness and good sensitivity in a population where antibody production is hampered. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Hospital São José de Doenças Infecciosas. Fortaleza, CE, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brasil. | pt_BR |
Subject | Histoplasmosis | pt_BR |
Subject | AIDS | pt_BR |
Subject | AIDS patients | pt_BR |
Subject | Diagnosis | pt_BR |
e-ISSN | 1439-0507 | pt_BR |
Peer reviewed | Não | pt_BR |
Publication status | Não publicado | pt_BR |