Author | Parente, T. E. M. | |
Author | Oliveira, A. C. A. X. de | |
Author | Beghini, D. G. | |
Author | Chapeaurouge, D. A. | |
Author | Perales, Jonas | |
Author | Paumgartten, F. J. R. | |
Access date | 2019-01-08T16:05:11Z | |
Available date | 2019-01-08T16:05:11Z | |
Document date | 2009 | |
Citation | PARENTE, T. E. M. et al. Lack of constitutive and inducible ethoxyresorufin-O-deethylase activity in the liver of suckermouth armored catfish (Hypostomus affinis and Hypostomus auroguttatus, Loricariidae). Comparative Biochemistry and Physiology, Part C, v.150, p.252–260, 2009. | pt_BR |
ISSN | 1532-0456 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/30928 | |
Language | eng | pt_BR |
Publisher | Elsevier | pt_BR |
Rights | restricted access | pt_BR |
Subject in Portuguese | Metabolismo xenobiótico | pt_BR |
Subject in Portuguese | tilápia do nilo | pt_BR |
Title | Lack of constitutive and inducible ethoxyresorufin-O-deethylase activity in the liver of suckermouth armored catfish (Hypostomus affinis and Hypostomus auroguttatus, Loricariidae) | pt_BR |
Type | Article | pt_BR |
DOI | 10.1016/j.cbpc.2009.05.006 | |
Abstract | We investigated the presence and inducibility of CYP1A in suckermouth catfish (Hypostomus affinis and Hypostomus auroguttatus, Loricariidae), tilapia (Oreochromis niloticus, Cichlidae) and mice (Mus musculus, Muridae). Alkoxyresorufin-O-dealkylases (EROD, MROD, PROD and BROD) were detected and proved to be inducible (beta-naphthoflavone, BNF or dimethylbenz[a]anthracene, DMBA, 50 mg/kg bw ip) in liver microsomes from tilapia and mice. In loricariids, alkoxyresorufin-O-dealkylases were either undetectable (MROD/EROD) or very low (PROD/BROD), and so they remained after treatment with BNF or DMBA. Ethoxycoumarin-O-deethylase (ECOD) was recorded in all species and proved not to be inducible by BNF or DMBA. In loricariids and tilapia, ECOD was not depressed by a concentration of alpha-naphthoflavone (CYP1A-inhibitor) that markedly depressed EROD in tilapia. A CYP1A-like protein was detected by a monoclonal antibody in rats, mice and tilapia, but not in loricariids. A polyclonal antibody, however, detected a CYP1A-like protein in liver microsomes of loricariids. Suckermouth catfish, rats, mice and tilapia express a protein reactive with a polyclonal antibody against trout CYP3A. Loricariids and tilapia exhibited marked genotoxic responses (enhanced incidence of micronucleated erythrocytes) following treatment DMBA (50 mg/kg bw ip), a promutagen activated by CYP1A/1B. Therefore, although not exhibiting EROD, a CYP1A-mediated activity, loricariids converted DMBA into its genotoxic metabolites. Our findings suggest that the CYP1A-like protein of locariid catfish recognizes DMBA, but not ethoxyresorufin, as a substrate. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Escola Nacional de Saúde Pública Sergio Arouca. Departamento de Ciências Biológicas. Laboratório de Toxicologia Ambiental. Rio de Janeiro, RJ. Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Escola Nacional de Saúde Pública Sergio Arouca. Departamento de Ciências Biológicas. Laboratório de Toxicologia Ambiental. Rio de Janeiro, RJ. Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxicologia. Rio de Janeiro, RJ. Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxicologia. Rio de Janeiro, RJ. Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxicologia. Rio de Janeiro, RJ. Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Escola Nacional de Saúde Pública Sergio Arouca. Departamento de Ciências Biológicas. Laboratório de Toxicologia Ambiental. Rio de Janeiro, RJ. Brasil. | pt_BR |
Subject | Cytochrome P450 | pt_BR |
Subject | Loricariidae | pt_BR |
Subject | Cichlidae | pt_BR |
Subject | EROD | pt_BR |
Subject | ECOD | pt_BR |
Subject | Oreochromis niloticus | pt_BR |
Subject | Xenobiotic metabolism | pt_BR |
Subject | Teleosts | pt_BR |
Subject | Nile tilapia | pt_BR |
DeCS | Sistema Enzimático do Citocromo P-450 | pt_BR |
DeCS | Ciclídeos | pt_BR |
DeCS | Citocromo P-450 CYP1A1 | pt_BR |
Embargo date | 2030-01-01 | |