| Author | Barbedo, Leonardo Silva | |
| Author | Figueiredo-Carvalho, Maria Helena Galdino | |
| Author | Muniz, Mauro de Medeiros | |
| Author | Zancopé-Oliveira, Rosely Maria | |
| Access date | 2019-01-24T11:18:48Z | |
| Available date | 2019-01-24T11:18:48Z | |
| Document date | 2017 | |
| Citation | BARBEDO, Leonardo Silva et al. Comparison of four molecular approaches to identify Candida parapsilosis complex species. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 113, n. 3, p. 214-219, Mar. 2017. | pt_BR |
| ISSN | 0074-0276 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/31274 | |
| Language | eng | pt_BR |
| Publisher | Instituto Oswaldo Cruz | pt_BR |
| Rights | open access | pt_BR |
| Title | Comparison of four molecular approaches to identify Candida parapsilosis complex species | pt_BR |
| Type | Article | pt_BR |
| DOI | 10.1590/0074-02760160412 | |
| Abstract | Since the description of Candida orthopsilosis and C. metapsilosis in 2005, several methods have been proposed to identify and differentiate these species from C. parapsilosis sensu stricto. Species-specific uniplex polymerase chain reaction (PCR) was performed and compared with sequencing of the D1/D2 region of the LSU 28S rDNA gene, microsatellite typing of C. parapsilosis sensu stricto, and PCR-restriction fragment length polymorphism patterns in the ITS1-5.8S-ITS2 region of the rDNA gene. There was agreement between results of testing of 98 clinical isolates with the four PCR-based methods, with 59 isolates identified as C. parapsilosis sensu stricto, 37 as C. orthopsilosis, and two as C. metapsilosis. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Candida parapsilosis complex | pt_BR |
| Subject | Candida orthopsilosis | pt_BR |
| Subject | Candida metapsilosis | pt_BR |
| Subject | Candidaemia | pt_BR |
| Subject | DNA | pt_BR |
| Subject | PCR | pt_BR |
| e-ISSN | 1678-8060 | |
