| Author | Lopes, Geovane Dias | |
| Author | Wiśniewski, Jacek R. | |
| Author | Souza, Nathalia Pinho de | |
| Author | Vidal, Vítor Ennes | |
| Author | Padrón, Gabriel | |
| Author | Britto, Constança | |
| Author | Cuervo, Patricia | |
| Author | Jesus, José Batista de | |
| Access date | 2019-01-24T12:57:05Z | |
| Available date | 2019-01-24T12:57:05Z | |
| Document date | 2018 | |
| Citation | LOPES, Geovane Dias; et al. In-Depth Quantitative Proteomic Analysis of Trophozoites and Pseudocysts of Trichomonas vaginalis. J. Proteome Res., v.17, p.3704-3718, 2018. | pt_BR |
| ISSN | 1535-3893 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/31284 | |
| Language | eng | pt_BR |
| Publisher | American Chemical Society | pt_BR |
| Rights | restricted access | |
| Subject in Portuguese | Trichomonas vaginalis | pt_BR |
| Subject in Portuguese | Pseudocisto | pt_BR |
| Subject in Portuguese | Ferro | pt_BR |
| Subject in Portuguese | Proteômica Quantitativa | pt_BR |
| Subject in Portuguese | Régua proteômica | pt_BR |
| Subject in Portuguese | Abordagem de proteína total | pt_BR |
| Title | In-Depth Quantitative Proteomic Analysis of Trophozoites and Pseudocysts of Trichomonas vaginalis | pt_BR |
| Type | Article | |
| DOI | 10.1021/acs.jproteome.8b00343 | |
| Abstract | Trichomonas vaginalis is a sexually transmitted anaerobic parasite that infects humans causing trichomoniasis, a common and ubiquitous sexually transmitted disease. The life cycle of this parasite possesses a trophozoite form without a cystic stage. However, the presence of nonproliferative and nonmotile, yet viable and reversible spherical forms with internalized flagella, denominated pseudocysts, has been commonly observed for this parasite. To understand the mechanisms involved in the formation of pseudocysts, we performed a mass spectrometry-based high-throughput quantitative proteomics study using a label-free approach and functional assays by biochemical and flow cytometric methods. We observed that the morphological transformation of trophozoite to pseudocysts is coupled to (i) a metabolic shift toward a less glycolytic phenotype; (ii) alterations in the abundance of hydrogenosomal iron-sulfur cluster (ISC) assembly machinery; (iii) increased abundance of regulatory particles of the ubiquitin-proteasome system; (iv) significant alterations in proteins involved in adhesion and cytoskeleton reorganization; and (v) arrest in G2/M phase associated with alterations in the abundance of regulatory proteins of the cell cycle. These data demonstrate that pseudocysts experience important physiological and structural alterations for survival under unfavorable environmental conditions. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Max-Planck-Institute for Biochemistry. Department of Proteomics and Signal Transduction. Martinsried, Germany. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Leishmaniose. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Estudos Integrados em Protozoologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Leishmaniose. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Leishmaniose. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ. Brasil / Universidade Federal de São João del Rei. Departamento de Medicina. São João del Rei, MG, Brasil. | pt_BR |
| Subject | Trichomonas vaginalis | pt_BR |
| Subject | Pseudocyst | pt_BR |
| Subject | Iron | pt_BR |
| Subject | FASP | pt_BR |
| Subject | Quantitative proteomics | pt_BR |
| Subject | Proteomic ruler | pt_BR |
| Subject | Total protein approach | pt_BR |
| e-ISSN | 1535-3907 | |
| Embargo date | 2030-01-01 | |
