| Author | Esteves, Leonardo Souza | |
| Author | Dalla Costa, Elis Regina | |
| Author | Vasconcellos, Sidra Ezidio Gonçalves | |
| Author | Vargas, Andrei | |
| Author | Ferreira Junior, Sérgio Luis Montego | |
| Author | Halon, Maria Laura | |
| Author | Ribeiro, Marta Osório | |
| Author | Rodenbusch, Rodrigo | |
| Author | Gomes, Harrison Magdinier | |
| Author | Suffys, Philip N. | |
| Author | Rossetti, Maria Lucia R. | |
| Access date | 2019-01-24T16:20:27Z | |
| Available date | 2019-01-24T16:20:27Z | |
| Document date | 2018 | |
| Citation | ESTEVES, Leonardo Souza; et al. Genetic diversity of Mycobacterium tuberculosis isoniazid monoresistant and multidrug-resistant in Rio Grande do Sul, a tuberculosis high-burden state in Brazil. Tuberculosis, v.110, p.36-43, 2018. | pt_BR |
| ISSN | 1472-9792 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/31303 | |
| Language | eng | pt_BR |
| Publisher | Elsevier | pt_BR |
| Rights | restricted access | |
| Subject in Portuguese | Tuberculose | pt_BR |
| Subject in Portuguese | Genotipagem | pt_BR |
| Subject in Portuguese | Resistência a Medicamentos | pt_BR |
| Subject in Portuguese | Brasil | pt_BR |
| Title | Genetic diversity of Mycobacterium tuberculosis isoniazid monoresistant and multidrug-resistant in Rio Grande do Sul, a tuberculosis high-burden state in Brazil | pt_BR |
| Type | Article | |
| DOI | 10.1016/j.tube.2018.02.009 | |
| Abstract | Tuberculosis (TB) remains a major public health problem in the world and Brazil is among the countries with the
highest incidence and prevalence rates, and Rio Grande do Sul, a Brazilian state, occupy a prominent position.
Multidrug-resistant Mycobacterium tuberculosis (MDR-TB) further aggravates this scenario, making it more difficult
to treat and control the disease. Isoniazid monoresistance (IMR) may increase the risk of progression to
MDR-TB and treatment failure. However, most drug resistance molecular tests only focus on detecting rifampicin
(RIF) resistance.In the present study, we characterized a total of 63 drug resistant isolates of M. tuberculosis (35
MDR, 26 IMR and two isolates monoresistant to rifampicin [RMR]) of the Rio Grande do Sul state by MIRUVNTR
(24 loci), spoligotyping, presence of RDRio, fbpC103, pks15/1 and sequencing of the katG, rpoB and inhA
genes. We observed a higher proportion of the LAM family 30/63 (47.61%). In IMR, mutations were found in the
katG gene (98% at codon 315) in 72.5%, and mutations in the promoter region of the inhA gene in 6.25% of the
isolates. In MDR-TB and RMR-TB isolates, 92.1% had mutations in the rpoB gene (57% at codon 531). The
presence of a 12 bp insertion between codons 516 and 517 of the rpoB gene in MDR-TB isolates was found in five
isolates.
In conclusion, we observed that the highest frequency of IMR-TB and MDR-TB strains belong to the LAM and
Haarlem genotypes in Rio Grande do Sul state. A significant number of isolates previously characterized as
Mycobacterium pinnipedi2 through spoligotyping were found to belong to the M. tuberculosis LAM family. This was
responsible for a number of significant cases and the molecular profile of this strain and the pattern of mutations
related to drug resistance were analyzed. These findings may contribute to a better understanding about the
spread of M. tuberculosis resistant in southern of Brazil. | pt_BR |
| Affilliation | Universidade Luterana do Brasil. Programa de Pós-graduação em Biologia Molecular e Celular Aplicado à Saúde. Canoas, RS, Brasil / Centro Estadual de Vigilância em Saúde. Secretaria Estadual de Saúde. Porto Alegre, RS, Brasil. / Universidade Federal do Rio de Janeiro. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Centro Estadual de Vigilância em Saúde. Secretaria Estadual de Saúde. Porto Alegre, RS, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular Aplicada a Micobactérias. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Centro Estadual de Vigilância em Saúde. Secretaria Estadual de Saúde. Porto Alegre, RS, Brasil. | pt_BR |
| Affilliation | Centro Estadual de Vigilância em Saúde. Secretaria Estadual de Saúde. Porto Alegre, RS, Brasil. | pt_BR |
| Affilliation | Centro Estadual de Vigilância em Saúde. Secretaria Estadual de Saúde. Porto Alegre, RS, Brasil. | pt_BR |
| Affilliation | Laboratório Central do Rio Grande do Sul. Porto Alegre, RS, Brasil. | pt_BR |
| Affilliation | Centro Estadual de Vigilância em Saúde. Secretaria Estadual de Saúde. Porto Alegre, RS, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular Aplicada a Micobactérias. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular Aplicada a Micobactérias. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Universidade Luterana do Brasil. Programa de Pós-graduação em Biologia Molecular e Celular Aplicado à Saúde. Canoas, RS, Brasil / Centro Estadual de Vigilância em Saúde. Secretaria Estadual de Saúde. Porto Alegre, RS, Brasil. / Universidade Federal do Rio de Janeiro. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Genotyping | pt_BR |
| Subject | Drug resistance | pt_BR |
| Subject | Tuberculosis | pt_BR |
| Subject | MIRU-VNTR | pt_BR |
| Subject | Brazil | pt_BR |
| e-ISSN | 1873-281X | |
| Embargo date | 2030-01-01 | |
| xmlui.metadata.dc.subject.ods | 03 Saúde e Bem-Estar | |
