| Author | Lauria, Pedro Santana Sales | |
| Author | Silva, Luciana Lyra Casais e | |
| Author | Espírito-Santo, Renan Fernandes do | |
| Author | Souza, Claudio Maurício Vieira de | |
| Author | Zingali, Russolina Benedeta | |
| Author | Caruso, Marjolly Brígido | |
| Author | Soares, Milena Botelho Pereira | |
| Author | Villarreal, Cristiane Flora | |
| Access date | 2019-01-25T18:20:42Z | |
| Available date | 2019-01-25T18:20:42Z | |
| Document date | 2018 | |
| Citation | LAURIA, P. S. S. et al. Pain-like behaviors and local mechanisms involved in the nociception experimentally induced by Latrodectus curacaviensis spider venom. Toxicology Letters,v. 299, p. 67–75, 2018. | pt_BR |
| ISSN | 0378-4274 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/31314 | |
| Sponsorship | Fundação de Amparo à Pesquisa do Estado da Bahia [grant number 11582/2015]. | pt_BR |
| Language | eng | pt_BR |
| Publisher | Elsevier | pt_BR |
| Rights | open access | pt_BR |
| Subject in Portuguese | Aranha viúva | pt_BR |
| Subject in Portuguese | Dor | pt_BR |
| Subject in Portuguese | Nocicepção | pt_BR |
| Subject in Portuguese | TRPV1 | pt_BR |
| Subject in Portuguese | Histamina | pt_BR |
| Title | Pain-like behaviors and local mechanisms involved in the nociception experimentally induced by Latrodectus curacaviensis spider venom | pt_BR |
| Type | Article | pt_BR |
| DOI | 10.1016/j.toxlet.2018.09.008 | |
| Abstract | The present study was undertaken to characterize the behavioral manifestations of nociception and the local mechanisms involved with the nociceptive response elicited by Latrodectus curacaviensis venom (LCV) in mice. After the intraplantar LCV inoculation, spontaneous nociception, mechanical and thermal nociceptive thresholds, motor performance, edema and cytokine levels were evaluated using von Frey filaments, hot/cold plate, rota-rod, plethismometer and ELISA, respectively. Analysis of LCV was performed by SDS-PAGE and chromatography. Intraplantar injection of LCV (1-100 ng/paw) induced intense and heat-sensitive spontaneous nociception, mediated by serotonin and bradykinin receptors, TRPV1 channels, as well as by transient local inflammation. LCV (0.1-10 ng/paw) induced mechanical allodynia, which was reduced by the local pretreatment with H1 receptor or TRPV1 antagonists. Corroborating the TRPV1 involvement, in thermal nociception assays, LCV induced a similar response to that of capsaicin, a TRPV1 agonist, facilitating the response to noxious hot stimuli and inhibiting the response to cold noxious stimulation. LCV promoted mast cell degranulation, increased IL-1β paw levels, but did not produce a relevant edematogenic effect. Analysis of LCV components showed a predominance of high molecular weight proteins. This work provides the first mechanistic hypothesis to explain the local pain induced by LCV, the most frequent clinical symptom of human envenomation. | pt_BR |
| Affilliation | Universidade Federal da Bahia. Faculdade de Farmácia. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Universidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Instituto Vital Brasil. Niterói, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Hospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Universidade Federal da Bahia. Faculdade de Farmácia. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
| Subject | Widow spider | pt_BR |
| Subject | Pain | pt_BR |
| Subject | Nociception | pt_BR |
| Subject | TRPV1 | pt_BR |
| Subject | Histamine | pt_BR |
