| Author | Azevedo-Pereira, Ricardo Luiz | |
| Author | Rangel, Bárbara | |
| Author | Tovar-Moll, Fernanda | |
| Author | Gasparetto, Emerson Leandro | |
| Author | Attias, Marcia | |
| Author | Zaverucha-do-Valle, Camila | |
| Author | Jasmin, null | |
| Author | Mendez-Otero, Rosalia | |
| Access date | 2019-01-29T12:18:11Z | |
| Available date | 2019-01-29T12:18:11Z | |
| Document date | 2018 | |
| Citation | AZEVEDO-PEREIRA, Ricardo Luiz et al. Superparamagnetic iron oxide nanoparticles as a tool to track mouse neural stem cells in vivo. Molecular Biology Reports, p. 1-8, 12 Nov. 2018. | pt_BR |
| ISSN | 0301-4851 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/31335 | |
| Language | eng | pt_BR |
| Publisher | Springer | pt_BR |
| Rights | restricted access | pt_BR |
| Title | Superparamagnetic iron oxide nanoparticles as a tool to track mouse neural stem cells in vivo | pt_BR |
| Type | Article | |
| DOI | 10.1007/s11033-018-4460-9 | |
| Abstract | Cell transplantation offers a promising approach in many neurological disorders. Neural stem (NS) cells are potential candidates for cell therapy. The ability to track the grafted cells in the host tissue will refine this therapy. Superparamagnetic iron oxide nanoparticles (SPION) have been suggested as a feasible method, but there is no consensus about its safety. Here we investigated the feasibility of label NS cells with SPION and track by MRI after transplantation into mouse striatum with SPION cells and its therapeutic effects by grafting the cells into mouse striatum. We demonstrated that SPION-labeled NS cells display normal patterns of cellular processes including proliferation, migration, differentiation and neurosphere formation. Transmission electron microscopy reveals SPION in the cytoplasm of the cells, which was confirmed by microanalysis. Neurons and astrocytes generated from SPION-labeled NS cells were able to carry nanoparticles after 7 days under differentiation. SPION-labeled NS cells transplanted into striatum of mice were detected by magnetic resonance imaging (MRI) and microscopy 51 days later. In agreement with others reports, we demonstrated that NS cells are able to incorporate SPION in vitro without altering the stemness, and can survive and be tracked by MRI after they have been grafted into mice striatum. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil / Stanford University. Department of Neurosurgery. Palo Alto, CA, USA. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Núcleo Multidisciplinar de Pesquisa em Biologia-Duque de Caxias. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Neural stem cells | pt_BR |
| Subject | Superparamagnetic iron oxide nanoparticle | pt_BR |
| Subject | Magnetic resonance imaging | pt_BR |
| Subject | Cellular therapy | pt_BR |
| Subject | Cell tracking | pt_BR |
| e-ISSN | 1573-4978 | |
