| Autor | Furtado, Marina C. | |
| Autor | Menezes, Rodrigo C. | |
| Autor | Kiupel, Matti | |
| Autor | Madeira, Maria F. | |
| Autor | Oliveira, Raquel V. C. | |
| Autor | Langohr, Ingeborg M. | |
| Autor | Figueiredo, Fabiano B. | |
| Data de acesso | 2019-02-05T12:48:29Z | |
| Data de disponibilização | 2019-02-05T12:48:29Z | |
| Data do publicação | 2015 | |
| Citação | FURTADO, Marina C. et al. Comparative study of in situ hybridization, immunohistochemistry and parasitological culture for the diagnosis of canine leishmaniosis. Parasites and Vectors, v. 8, n. 620, p. 1-5, 2015. | pt_BR |
| ISSN | 1756-3305 | |
| URI | https://www.arca.fiocruz.br/handle/icict/31416 | |
| Idioma | eng | pt_BR |
| Editor | BMC | pt_BR |
| Direito Autoral | open access | pt_BR |
| Título | Comparative study of in situ hybridization, immunohistochemistry and parasitological culture for the diagnosis of canine leishmaniosis | pt_BR |
| Tipo do documento | Article | pt_BR |
| DOI | 10.1186/s13071-015-1224-4 | |
| Resumo em Inglês | Background: The establishment of an accurate diagnostic protocol for canine visceral leishmaniosis (CanL) is a
significant laboratory challenge and the lack of a reliable reference standard is one of the major problems. The aim of this study was to compare in situ hybridization (ISH), immunohistochemistry (IHC) and parasitological culture (PC) for detection of L. infantum in skin, spleen, lymph node and bone marrow of clinically healthy and sick seropositive dogs.
Findings: The study included 65 dogs positive with both DPP® and ELISA for anti-Leishmania antibodies. In situ hybridization of spleen or lymph node had the highest positivity rates of L. infantum detection. The total positivity rates for IHC, ISH and PC were 70 %, 68.1 % and 65.8 %, respectively. When combining techniques, the positivity rates were 81.5 % in the spleen, 79.0 % in lymph nodes, 59.0 % in bone marrow and 52.3 % in the skin. The highest percentage of infected dogs (87.7 %) was detected by using lymph node samples. When examining only skin, positivity was significantly higher in sick dogs than in the clinically healthy dogs. Infection with L. infantum was confirmed in 95.8 % of sick dogs and in 82.4 % of healthy dogs.
Conclusions: Considering the advantages of accurately diagnosing different Leishmania species and of being more sensitive than PC, ISH should be considered as reference standard test for the diagnosis of CanL. Spleen and lymph node are the most suitable tissues to confirm infection with L. infantum in seropositive dogs. The testing of only skin from clinically healthy dogs may result in a high percentage of false negative results. | pt_BR |
| Afiliação | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em Dermatozoonoses em Animais Domésticos. Rio de Janeiro, RJ, Brasil / Michigan State University. College of Veterinary Medicine. Department of Pathobiology and Diagnostic Investigation. Lansing, MI, USA. | pt_BR |
| Afiliação | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em Dermatozoonoses em Animais Domésticos. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Afiliação | Michigan State University. College of Veterinary Medicine. Department of Pathobiology and Diagnostic Investigation. Lansing, MI, USA. | pt_BR |
| Afiliação | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Vigilância em Leishmanioses. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Afiliação | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Epidemiologia Clínica. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Afiliação | Michigan State University. College of Veterinary Medicine. Department of Pathobiology and Diagnostic Investigation. Lansing, MI, USA / Louisiana State University. School of Veterinary Medicine. Department of Pathobiological Sciences. Baton Rouge, LA, USA. | pt_BR |
| Afiliação | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em Dermatozoonoses em Animais Domésticos. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Palavras-chave em inglês | Leishmania infantum | pt_BR |
| Palavras-chave em inglês | Dog | pt_BR |
| Palavras-chave em inglês | Diagnosis | pt_BR |
| Palavras-chave em inglês | In situ hybridization | pt_BR |
| Palavras-chave em inglês | Immunohistochemistry | pt_BR |
| Palavras-chave em inglês | Parasitological culture | pt_BR |
| e-ISSN | 1756-3305 | |
