Author | Rusak, Leonardo Alves | |
Author | Pereira, Rodrigo de Castro Lisboa | |
Author | Freitag, Isabelle Geoffroy | |
Author | Hofer, Cristina Barroso | |
Author | Hofer, Ernesto | |
Author | Asensi, Marise Dutra | |
Author | Vallim, Deyse Christina | |
Access date | 2019-02-06T15:32:50Z | |
Available date | 2019-02-06T15:32:50Z | |
Document date | 2018 | |
Citation | RUSAK. Leonardo Alves; et al. Rapid detection of Yersinia enterocolitica serotype O:3 using a duplex PCR assay. Journal of Microbiological Methods, v.154, p.107-11, Oct. 2018. | pt_BR |
ISSN | 0167-7012 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/31460 | |
Language | eng | pt_BR |
Publisher | Elsevier | pt_BR |
Rights | restricted access | |
Subject in Portuguese | Reação em Cadeia da Polimerase | pt_BR |
Subject in Portuguese | Detecção | pt_BR |
Subject in Portuguese | Yersinia enterocolitica | pt_BR |
Subject in Portuguese | Método de triagem | pt_BR |
Title | Rapid detection of Yersinia enterocolitica serotype O:3 using a duplex PCR assay | pt_BR |
Type | Article | |
DOI | 10.1016/j.mimet.2018.10.014 | |
Abstract | Yersinia enterocolitica, a member of the Enterobacteriaceae family, is a zoonotic agent that causes gastrointestinal diseases and some extraintestinal disorders in humans. Y. enterocolitica ssp. palearctica bioserotype 4/O:3 is the primary pathogenic bioserotype in Europe, where it has a high public health relevance. The isolation and identification of Y. enterocolitica from various sources on selective media have been seldom successful due to several reasons. In an attempt to overcome the problems associated with traditional culture-based methods, we developed a single duplex PCR assay for the detection of Y. enterocolitica ssp. palearctica bioserotype 4/O:3 using DNA extracted from a source. We combined the primer for tufA (elongation factor Tu) with the primer for rfbC (the biosynthesis of the O side chain) in one single reaction, which showed good results when we analyzed 88 Yersinia strains and when it was tested in the DNA from stool samples of two groups of pregnant women, one comprising HIV-positive women and the other comprising of HIV-negative women. Furthermore, the duplex PCR assay was found to be 16 times better in detecting Yersinia spp. in stool samples than the culture-based method. In addition, it was found to be a rapid screening method for the detection of Y. enterocolitica serotype O:3, and it could still detect other Y. enterocolitica serotypes and Yersinia species as well. We anticipate that the duplex PCR assay could be a useful tool for hospital and veterinary surveillance studies on Yersinia worldwide. | pt_BR |
Affilliation | Fundação Oswaldo Cruz, Instituto Oswaldo Cruz, Laboratório de Pesquisa em Infecção Hospitalar, Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz, Instituto Oswaldo Cruz, Laboratório de Zoonoses Bacterianas. Setor Listeria, Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz, Instituto Oswaldo Cruz, Laboratório de Zonoses Bacterianas. Setor Listeria. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz, Instituto Oswaldo Cruz, Laboratório de Zoonoses Bactrianas. Setor Listeria. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Faculdade de Medicina. Programa de Pós-Graduação em Doenças Infecciosas e Parasitárias. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Universidade Federal do Rio de Janeiro. Faculdade de Medicina. Programa de Pó-Graduação em Doenças Infecciosas e Parasitárias. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz, Instituto Oswaldo Cruz, Laboratório de Zoonoses Bacterianas.. Setor Listeria. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz, Instituto Oswaldo Cruz, Laboratório de Pesquisa em Infecção Hospitalar, Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz, Instituto Oswaldo Cruz, Laboratório de Zoonoses Bacterianas. Setor Listeria, Rio de Janeiro, RJ, Brasil | pt_BR |
Subject | Detection | pt_BR |
Subject | PCR | pt_BR |
Subject | Screening method | pt_BR |
Subject | Yersinia enterocolitica O:3 | pt_BR |
Subject | Yersinia spp | pt_BR |
e-ISSN | 1872-8359 | |
Embargo date | 2030-01-01 | |