| Author | Lima, Luana Nepomueceno Costa | |
| Author | Frota, Cristiane Cunha | |
| Author | Suffys, Phillip Noel | |
| Author | Fontes, Amanda Nogueira Brum | |
| Author | Mota, Rosa Maria Salani | |
| Author | Almeida, Rosa Livia Freitas | |
| Author | Pontes, Maria Araci de Andrade | |
| Author | Gonçalves, Heitor de Sá | |
| Author | Kendall, Carl | |
| Author | Kerr, Ligia Regina Sansigolo | |
| Access date | 2019-02-07T13:30:47Z | |
| Available date | 2019-02-07T13:30:47Z | |
| Document date | 2018 | |
| Citation | LIMA, Luana Nepomuceno Costa et al. Genotyping comparison of Mycobacterium leprae isolates by VNTR analysis from nasal samples in a Brazilian endemic region. Pathogens and Global Health, v. 112, n. 2, p. 79-85, 2018. | pt_BR |
| ISSN | 2047-7724 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/31476 | |
| Language | eng | pt_BR |
| Publisher | Taylor & Francis | pt_BR |
| Rights | restricted access | |
| Subject in Portuguese | Hanseníase | pt_BR |
| Subject in Portuguese | Mycobacterium leprae | pt_BR |
| Subject in Portuguese | Cavidade nasal | pt_BR |
| Subject in Portuguese | Genótipo | pt_BR |
| Subject in Portuguese | Marcador genético | pt_BR |
| Subject in Portuguese | Análise de cluster | pt_BR |
| Title | Genotyping comparison of Mycobacterium leprae isolates by VNTR analysis from nasal samples in a Brazilian endemic region | pt_BR |
| Type | Article | |
| DOI | 10.1080/20477724.2018.1427308 | |
| Abstract | This study analyzed the genetic diversity by MIRU-VNTR of Mycobacterium leprae isolates from nasal cavities and related to epidemiological and clinical data. The sample consisted of 48 newly diagnosed leprosy cases that tested positive for M. leprae PCR in nasal secretion (NS) attending to the National Reference Center of Dermatology Dona Libania (CDERM), Fortaleza, Brazil. Total DNA was extracted from NS of each patient and used for amplification of four M. leprae VNTR loci. Four clusters of M. leprae isolates were formed with identical genotypes. In the spatial analysis, 12 leprosy cases presented similar genotypes organized into 4 clusters. The most common genotypes in the current study was AC8b: 8, AC9: 7, AC8a: 8, GTA9: 10, which may represent a genotype of circulating strains most often in Ceará. A minimum set of four MIRU-VNTR loci was demonstrated to study the genetic diversity of M. leprae isolates from NS. | pt_BR |
| Affilliation | Universidade Federal do Ceará. Faculdade de Medicina. Departamento de Patologia e Medicina Legal. Fortaleza, CE, Brasil / Instituto Evandro Chagas. Seção de Bacteriologia e Micologia. Ananindeua, PA, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Ceará. Faculdade de Medicina. Departamento de Patologia e Medicina Legal. Fortaleza, CE, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular Aplicada a Micobactérias. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular Aplicada a Micobactérias. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Ceará. Faculdade de Medicina. Departamento de Estatística e Matemática aplicada. Fortaleza, CE, Brasil. | pt_BR |
| Affilliation | Universidade de Fortaleza. Programa de Pós-graduação em Saúde Coletiva. Fortaleza, CE, Brasil. | pt_BR |
| Affilliation | Departamento do Estado do Ceará. Centro de Dermatologia Dona Libânia. Fortaleza, CE, Brasil. | pt_BR |
| Affilliation | Departamento do Estado do Ceará. Centro de Dermatologia Dona Libânia. Fortaleza, CE, Brasil. | pt_BR |
| Affilliation | tulane University. School of Public Health and Tropical Medicine. Department of Global Community Health and Behavioral Sciences. New Orleans, LA, USA. | pt_BR |
| Affilliation | Universidade Federal do Ceará. Faculdade de Medicina. Departamento de Saúde Comunitária. Fortaleza, CE, Brasil. | pt_BR |
| Subject | Leprosy | pt_BR |
| Subject | Mycobacterium leprae | pt_BR |
| Subject | Brazil | pt_BR |
| Subject | Nasal cavity | pt_BR |
| Subject | Genotype | pt_BR |
| Subject | Genetic marker | pt_BR |
| Subject | VNTR loci | pt_BR |
| Subject | Cluster analysis | pt_BR |
| e-ISSN | 2047-7732 | |
| Embargo date | 2030-01-01 | |
