| Author | Santos, C. Rocha | |
| Author | Bastos, F. F. | |
| Author | Dantas, R. F. | |
| Author | Hauser-Davis, R. A. | |
| Author | Rodrigues, L. C. | |
| Author | Cunha Bastos, V. L. F. | |
| Author | Bastos, J. Cunha | |
| Access date | 2019-02-07T13:50:50Z | |
| Available date | 2019-02-07T13:50:50Z | |
| Document date | 2018 | |
| Citation | SANTOS, C. Rocha; et al. Glutathione peroxidase and glutathione S-transferase in blood and liver from a hypoxia-tolerant fish under oxygen deprivation. Ecotoxicology and Environmental Safety, v.163, p.604-611, Aug. 2018. | pt_BR |
| ISSN | 0147-6513 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/31478 | |
| Language | eng | pt_BR |
| Publisher | Elsevier | pt_BR |
| Rights | restricted access | |
| Subject in Portuguese | Biomarcadores | pt_BR |
| Subject in Portuguese | Glutationa Peroxidase | pt_BR |
| Subject in Portuguese | Glutationa Transferase | pt_BR |
| Subject in Portuguese | Hipóxia | pt_BR |
| Title | Glutathione peroxidase and glutathione S-transferase in blood and liver from a hypoxia-tolerant fish under oxygen deprivation | pt_BR |
| Type | Article | |
| DOI | 10.1016/j.ecoenv.2018.06.089 | |
| Abstract | Liver enzyme activities can be employed as biomarkers, but liver can only be obtained with death of the specimen. On the other hand, blood withdrawal is a non-lethal procedure. Accordingly, the hypothesis of this study is to verify if glutathione peroxidase (GPX) and glutathione S-transferase (GST) activities in blood parallel those in the liver of the hypoxia-tolerant fish, Piaractus mesopotamicus (pacu), submitted to hypoxia conditions. GPX was assayed with H2O2 in cytosols from both liver and erythrocytes and exhibited no significant variation, either in erythrocytes or in liver, when comparing pacus under normoxia with those under hypoxia (42 h). GST activity with chloro-dinitrobenzene (CDNB), an artificial substrate suitable for almost all GST isoenzymes, was compared to activity with 4-hydroxy-nonenal (4-HNE), a physiological endogenous substrate. GST activity with CDNB did not change in liver or in erythrocyte cytosols in pacus under hypoxia compared to those under normoxia. On the other hand, a significant decrease in erythrocyte activity with 4-HNE was observed after 42 h of hypoxia in both erythrocytes and liver, which may be a response to increased lipid oxidation in erythrocytes. Erythrocyte GST activity was 3-fold higher with 4-HNE than with CDNB, indicating that 4-HNE is a more appropriate substrate to determine GST activity in pacu erythrocytes. | pt_BR |
| Affilliation | Universidade do Estado do Rio de Janeiro. Instituto de Biologia Roberto Alcântara Gomes. Departamento de Bioquímica. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio deJaneiro. Centro de Ciências da Saúde. Instituto de Bioquímica Leopoldo de Meis. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade do Estado do Rio de Janeiro. Instituto de Biologia Roberto Alcântara Gomes. Departamento de Bioquímica. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade do Estado do Rio de Janeiro. Instituto de Biologia Roberto Alcântara Gomes. Departamento de Bioquímica. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica de Proteínas e Peptídeos. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Escola Nacional de Saúde Pública Sergio Arouca. Centro de Estudos da Saúde do Trabalhador e Ecologia Humana. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade do Estado do Rio de Janeiro. Instituto de Biologia Roberto Alcântara Gomes. Departamento de Bioquímica. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade do Estado do Rio de Janeiro. Instituto de Biologia Roberto Alcântara Gomes. Departamento de Bioquímica. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade do Estado do Rio de Janeiro. Instituto de Biologia Roberto Alcântara Gomes. Departamento de Bioquímica. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | 4-hydroxy-nonenal | pt_BR |
| Subject | Biomarkers | pt_BR |
| Subject | Glutathione peroxidase | pt_BR |
| Subject | Glutathione S-transferase | pt_BR |
| Subject | Hypoxia | pt_BR |
| Subject | Piaractus mesopotamicus | pt_BR |
| Embargo date | 2030-01-01 | |
