| Author | Vazquez, Leonardo | |
| Author | Ferreira, Andre Teixeira da Silva | |
| Author | Cavalcante, Fernanda Sampaio | |
| Author | Garcia, Israel José | |
| Author | Santos, Katia Regina Netto dos | |
| Author | Barbosa, Leonardo Augusto de Oliveira | |
| Author | Almeida, Marcius da Silva | |
| Author | Mignaco, Julio Alberto | |
| Author | Fontes, Carlos Frederico Leite | |
| Access date | 2019-02-09T13:17:57Z | |
| Available date | 2019-02-09T13:17:57Z | |
| Document date | 2018 | |
| Citation | VAZQUEZ, Leonardo; et al. Properties of novel surfactin-derived biosurfactants obtained through solid-phase synthesis. J. Pep. Sci., v.24, e3129, 13p, 2018. | pt_BR |
| ISSN | 1075-2617 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/31510 | |
| Language | eng | pt_BR |
| Publisher | Wiley | pt_BR |
| Rights | restricted access | |
| Subject in Portuguese | Biosurfactantes | pt_BR |
| Subject in Portuguese | Entrega de drogas | pt_BR |
| Subject in Portuguese | Peptídeos lineares | pt_BR |
| Subject in Portuguese | Lipopeptídeos | pt_BR |
| Subject in Portuguese | Peptídeos | pt_BR |
| Subject in Portuguese | Design racional | pt_BR |
| Subject in Portuguese | Síntese em fase sólida | pt_BR |
| Subject in Portuguese | Efeito sinérgico | pt_BR |
| Subject in Portuguese | Surfactina | pt_BR |
| Title | Properties of novel surfactin-derived biosurfactants obtained through solid-phase synthesis | pt_BR |
| Type | Article | |
| DOI | 10 .1002/psc.3129 | |
| Abstract | Eight molecules, four peptides (SPs) and four lipopeptides (LPs) derived by rational design from surfactin, a well-known secreted biosurfactant from Bacillus subtilis, were produced employing Fmoc-based solid-phase synthesis. These new peptides were tested to evaluate their potential biosurfactant and biological activities, aiming at possible applications in industrial, biological, pharmaceutical, and medical use. Five molecules (SP1, SP2, SP4, LP5, and LP8) presented potential for medical uses, mainly due to their drug delivery properties as suggested by their synergistic activity with the antibiotic vancomycin against Staphylococcus aureus. All synthetic peptides showed low toxicity against Vero cell cultures, in assays of hemolysis, and in different cytotoxicity assays. In addition, we found that three peptides (SP1, LP6, and LP7) had potential technological and industrial use because of their emulsifying capacity, low toxicity, and ability to physically stabilize solutions. These novel molecules retained some properties of the parental molecule (surfactin, which was originally obtained through nonribosomal synthesis in Bacillus subtilis) but have the advantage of being linear peptides, which can be produced at large scales through the use of conventional heterologous protein expression protocols. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis. Laboratório Est. e Reg. de Proteínas e ATPaes. Programa de Biologia Estrutural. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Campus de Macaé. Departamento de Microbiologia. Macaé, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal de São João del Rei. Departamento de Bioquímica. Laboratório de Bioquímica Celular. São João del Rei, MG, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal de São João del Rei. Departamento de Bioquímica. Laboratório de Bioquímica Celular. São João del Rei, MG, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Centro Nacional de Biologia Estrutural e Bioimagem. Programa de Biologia Estrutural. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis. Laboratório Est. e Reg. de Proteínas e ATPaes. Programa de Biologia Estrutural. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis. Laboratório Est. e Reg. de Proteínas e ATPaes. Programa de Biologia Estrutural. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Biosurfactants | pt_BR |
| Subject | Drug delivery | pt_BR |
| Subject | Linear peptides | pt_BR |
| Subject | Lipopeptides | pt_BR |
| Subject | Peptide | pt_BR |
| Subject | Rational design | pt_BR |
| Subject | Solid-phase synthesis | pt_BR |
| Subject | Surfactin | pt_BR |
| Subject | Synergetic effext | pt_BR |
| e-ISSN | 1099-1387) | |
| Embargo date | 2030-01-01 | |
