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| Autor | Almeida, Fernanda | |
| Autor | Seribelli, Amanda Aparecida | |
| Autor | Medeiros, Marta Inês Cazentini | |
| Autor | Rodrigues, Dália dos Prazeres | |
| Autor | Varani, Alessandro de Mello | |
| Autor | Luo, Yan | |
| Autor | Allard, Marc W. | |
| Autor | Falcão, Juliana Pfrimer | |
| Fecha de acceso | 2019-02-12T10:56:00Z | |
| Fecha de disponibilización | 2019-02-12T10:56:00Z | |
| Fecha de publicación | 2018 | |
| Referencia | ALMEIDA, Fernanda; et al. Phylogenetic and antimicrobial resistance gene analysis of Salmonella Typhimurium strains isolated in Brazil by whole genome sequencing. Plos One, v.13, n.8, e0201882, 16p, Aug. 2018. | pt_BR |
| ISSN | 1932-6203 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/31552 | |
| Idioma | eng | pt_BR |
| Editor | Public Library of Science | pt_BR |
| Derechos de autor | open access | |
| Palabras clave en Portugués | Sequenciamento Completo do Genoma | pt_BR |
| Palabras clave en Portugués | Cepas | pt_BR |
| Palabras clave en Portugués | Salmonella Typhimurium | pt_BR |
| Palabras clave en Portugués | Resistência antimicrobiana | pt_BR |
| Palabras clave en Portugués | Filogenética | pt_BR |
| Palabras clave en Portugués | Análise genética | pt_BR |
| Palabras clave en Portugués | Brasil | pt_BR |
| Título | Phylogenetic and antimicrobial resistance gene analysis of Salmonella Typhimurium strains isolated in Brazil by whole genome sequencing | pt_BR |
| Tipo del documento | Article | |
| DOI | 10.1371/journal.pone.0201882 | |
| Resumen en Inglés | Whole genome sequencing (WGS) has been used as a powerful technology for molecular epidemiology, surveillance, identification of species and serotype, identification of the sources of outbreaks, among other purposes. In Brazil, there is relatively few epidemiological data on Salmonella. In this study, 90 Salmonella Typhimurium strains had their genome sequenced to uncover the diversity of Salmonella Typhimurium isolated from humans and food, between 1983 and 2013, from different geographic regions in Brazil based on single nucleotide polymorphism (SNP) analysis. A total of 39 resistance genes were identified, such as aminoglycoside, tetracycline, sulfonamide, trimethoprim, beta-lactam, fluoroquinolone, phenicol and macrolide, as well as the occurrence of point mutations in some of the genes such as gyrA, gyrB, parC and parE. A total of 65 (72.2%) out of 90 S. Typhimurium strains studied were phenotypically resistant to sulfonamides, 44 (48.9%) strains were streptomycin resistant, 27 (30%) strains were resistant to tetracycline, 21 (23.3%) strains were gentamicin resistant, and seven (7.8%) strains were resistant to ceftriaxone. In the gyrA gene, it was observed the following amino acid substitutions: Asp(87)→Gly, Asp(87)→Asn, Ser(83)→Phe, Ser(83)→Tyr. Phylogenetic results placed the 90 S. Typhimurium strains into two major clades suggesting the existence of a prevalent subtype, likely more adapted, among strains isolated from humans, with some diversity in subtypes in foods. The variety and prevalence of resistant genes found in these Salmonella Typhimurium strains reinforces their potential hazard for humans and the risk in foods in Brazil. | pt_BR |
| Afiliación | Universidade de São Paulo. Faculdade de Ciências Farmacêuticas de Ribeirão Preto. Departamento de Análises Clínicas, Toxicológicas e Bromatológicas. Ribeirão Preto, SP, Brasil. | pt_BR |
| Afiliación | Universidade de São Paulo. Faculdade de Ciências Farmacêuticas de Ribeirão Preto. Departamento de Análises Clínicas, Toxicológicas e Bromatológicas. Ribeirão Preto, SP, Brasil. | pt_BR |
| Afiliación | Instituto Adolfo Lutz. Centro de Laboratório Regional de Ribeirão Preto. Ribeirão Preto, SP, Brasil. | pt_BR |
| Afiliación | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Enterobactérias. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Afiliación | Universidade Estadual Paulista. Faculdade de Ciências Agrárias e Veterinárias. Jaboticabal, SP, Brasil.. | pt_BR |
| Afiliación | U.S. Food and Drug Administration. Center for Food Safety and Applied Nutrition. Division of Microbiology, Office of Regular Science. College Park, Maryland, USA. | pt_BR |
| Afiliación | U.S. Food and Drug Administration. Center for Food Safety and Applied Nutrition. Division of Microbiology, Office of Regular Science. College Park, Maryland, USA. | pt_BR |
| Afiliación | Universidade de São Paulo. Faculdade de Ciências Farmacêuticas de Ribeirão Preto. Departamento de Análises Clínicas, Toxicológicas e Bromatológicas. Ribeirão Preto, SP, Brasil. | pt_BR |
| Palavras clave en Inglês | Salmonella Typhimurium | pt_BR |
| Palavras clave en Inglês | Strains | pt_BR |
| Palavras clave en Inglês | Whole Genome Sequencing | pt_BR |
| Palavras clave en Inglês | Antimicrobial resistance | pt_BR |
| Palavras clave en Inglês | Phylogenetic | pt_BR |
| Palavras clave en Inglês | Gene analysis | pt_BR |
| Palavras clave en Inglês | Brazil | pt_BR |
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