| Author | Silveira, Melise Chaves | |
| Author | Silva, Rangeline Azevedo da | |
| Author | Mota, Fábio Faria da | |
| Author | Catanho, Marcos | |
| Author | Jardim, Rodrigo | |
| Author | Guimarães, Ana Carolina R. | |
| Author | Miranda, Antonio B. de | |
| Access date | 2019-02-13T16:27:43Z | |
| Available date | 2019-02-13T16:27:43Z | |
| Document date | 2018 | |
| Citation | SILVEIRA, Melise Chaves; et al. Systematic Identification and Classification of β-Lactamases Based on Sequence Similarity Criteria: β-Lactamase Annotation. Evolutionary Bioinformatics, v.14, p.1-11, 2018. | pt_BR |
| ISSN | 1176-9343 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/31608 | |
| Language | eng | pt_BR |
| Publisher | SAGE Publications | pt_BR |
| Rights | open access | |
| Subject in Portuguese | β-lactamase | pt_BR |
| Subject in Portuguese | Classe | pt_BR |
| Subject in Portuguese | Sub-classe | pt_BR |
| Subject in Portuguese | Identificação | pt_BR |
| Subject in Portuguese | Semelhança de sequência | pt_BR |
| Title | Systematic Identification and Classification of β-Lactamases Based on Sequence Similarity Criteria: β-Lactamase Annotation | pt_BR |
| Type | Article | |
| DOI | 10.1177/1169343118797351 | |
| Abstract | β-lactamases, the enzymes responsible for resistance to β-lactam antibiotics, are widespread among prokaryotic genera. However, current β-lactamase classification schemes do not represent their present diversity. Here, we propose a workflow to identify and classify βlactamases. Initially, a set of curated sequences was used as a model for the construction of profiles Hidden Markov Models (HMM), specific for each β-lactamase class. An extensive, nonredundant set of β-lactamase sequences was constructed from 7 different resistance proteins databases to test the methodology. The profiles HMM were improved for their specificity and sensitivity and then applied to fully assembled genomes. Five hierarchical classification levels are described, and a new class of β-lactamases with fused domains is proposed. Our profiles HMM provide a better annotation of β-lactamases, with classes and subclasses defined by objective criteria such as sequence similarity. This classification offers a solid base to the elaboration of studies on the diversity, dispersion, prevalence, and evolution of the different classes and subclasses of this critical enzymatic activity. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Computacional e Sistemas. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Computacional e Sistemas. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Computacional e Sistemas. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genômica Funcional e Bioinformática. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Computacional e Sistemas. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genômica Funcional e Bioinformática. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Computacional e Sistemas. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | β-lactamase | pt_BR |
| Subject | Class | pt_BR |
| Subject | Subclass | pt_BR |
| Subject | Identification | pt_BR |
| Subject | Sequence similarity | pt_BR |
