| Author | Silva, Andrea Martins da | |
| Author | Telleria, Erich Loza | |
| Author | Batista, Michel | |
| Author | Marchini, Fabricio Klerynton | |
| Author | Traub-Csekö, Yara Maria | |
| Author | Tempone, Antonio Jorge | |
| Access date | 2019-02-14T13:52:25Z | |
| Available date | 2019-02-14T13:52:25Z | |
| Document date | 2018 | |
| Citation | SILVA, Andrea Martins da; et al. Identification of Secreted Proteins Involved in Nonspecific dsRNA-Mediated Lutzomyia longipalpis LL5 Cell Antiviral Response. Viruses, v.1, n.43, 17p, Jan. 2018. | pt_BR |
| ISSN | 1999-4915 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/31641 | |
| Language | eng | pt_BR |
| Publisher | MDPI | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Lutzomyia longipalpis | pt_BR |
| Subject in Portuguese | Resposta antiviral | pt_BR |
| Subject in Portuguese | Resposta do tipo interferon | pt_BR |
| Subject in Portuguese | Células Embrionárias LL5 | pt_BR |
| Title | Identification of Secreted Proteins Involved in Nonspecific dsRNA-Mediated Lutzomyia longipalpis LL5 Cell Antiviral Response | pt_BR |
| Type | Article | |
| DOI | 10.3390/v10010043 | |
| Abstract | Hematophagous insects transmit infectious diseases. Sand flies are vectors of leishmaniasis,
but can also transmit viruses. We have been studying immune responses of Lutzomyia longipalpis,
the main vector of visceral leishmaniasis in the Americas. We identified a non-specific antiviral
response in L. longipalpis LL5 embryonic cells when treated with non-specific double-stranded RNAs
(dsRNAs). This response is reminiscent of interferon response in mammals. We are investigating
putative effectors for this antiviral response. Secreted molecules have been implicated in immune
responses, including interferon-related responses. We conducted a mass spectrometry analysis of
conditioned medium from LL5 cells 24 and 48 h after dsRNA or mock treatment. We identified
304 proteins. At 24 h, 19 proteins had an abundance equal or greater than 2-fold change, while
the levels of 17 proteins were reduced when compared to control cells. At the 48 h time point,
these numbers were 33 and 71, respectively. The two most abundant secreted peptides at 24 h in
the dsRNA-transfected group were phospholipid scramblase, an interferon-inducible protein that
mediates antiviral activity, and forskolin-binding protein (FKBP), a member of the immunophilin
family, which mediates the effect of immunosuppressive drugs. The transcription profile of most
candidates did not follow the pattern of secreted protein abundance. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Parasitas e Vetores. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Parasitas e Vetores. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Genômica Funcional. Curitiba, PR, Brasil / Fundação Oswaldo Cruz. Instituto Carlos Chagas. Plataforma Espectrometria de Massas. Curitiba, PR, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Genômica Funcional. Curitiba, PR, Brasil / Fundação Oswaldo Cruz. Instituto Carlos Chagas. Plataforma Espectrometria de Massas. Curitiba, PR, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Parasitas e Vetores. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Parasitas e Vetores. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Subject | Lutzomyia longipalpis | pt_BR |
| Subject | Embryonic LL5 cells | pt_BR |
| Subject | Interferon-like response | pt_BR |
| Subject | Poly (I:C) | pt_BR |
| Subject | Exoproteome | pt_BR |
| DeCS | Poli C | pt_BR |
| DeCS | Poli I | pt_BR |
| e-ISSN | 1999-4915 | |
