| Author | Mazur, Karin Luize | |
| Author | Feuser, Paulo Emílio | |
| Author | Valério, Alexsandra | |
| Author | Cordeiro, Arthur Poester | |
| Author | Oliveira, Camila Indiani de | |
| Author | Assolini, João Paulo | |
| Author | Pavanelli, Wander Rogério | |
| Author | Sayer, Claudia | |
| Author | Araújo, Pedro H. H | |
| Access date | 2019-02-14T14:06:33Z | |
| Available date | 2019-02-14T14:06:33Z | |
| Document date | 2018 | |
| Citation | MAZUR, K. L. et al. Diethyldithiocarbamate loaded in beeswax-copaiba oil nanoparticles obtained by solventless double emulsion technique promote promastigote death in vitro. Colloids and Surfaces B: Biointerfaces, v. 176, p. 507–512, 2019. | pt_BR |
| ISSN | 0927-7765 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/31644 | |
| Sponsorship | CAPES−Coordenação de
Aperfeiçoamento de Pessoal de Nível Superior Tecnológico and CNPq -
Conselho Nacional de Desenvolvimento Científico e Tecnológico and
Laboratório Central de Microscopia Eletrônica (LCME) of Federal
University of Santa Catarina for the TEM analyses. | pt_BR |
| Language | eng | pt_BR |
| Publisher | Elsevier | pt_BR |
| Rights | open access | pt_BR |
| Subject in Portuguese | Nanopartículas lipídicas sólidas | pt_BR |
| Subject in Portuguese | Dietilditiocarbamato | pt_BR |
| Subject in Portuguese | Drogas Hidrofílicas | pt_BR |
| Subject in Portuguese | Cera de Abelha | pt_BR |
| Subject in Portuguese | L. amazonensis | pt_BR |
| Title | Diethyldithiocarbamate loaded in beeswax-copaiba oil nanoparticles obtained by solventless double emulsion technique promote promastigote death in vitro | pt_BR |
| Type | Article | pt_BR |
| DOI | 10.1016/j.colsurfb.2018.12.048 | |
| Abstract | Leishmaniasis is considered a neglected tropical disease that represents a Public Health problem due to its high incidence. In the search of new alternatives for Leishmaniasis treatment diethyldithiocarbamate (DETC) has shown an excellent leishmanicidal activity and the incorporation into drug carrier systems, such as solid lipid nanoparticles (SLNs), is very promising. In the present work DETC loaded in beeswax nanoparticles containing copaiba oil were obtained by the double emulsion/melt technique. The nanoparticles were characterized and leishmanicidal activity against L. amazonensis promastigotes forms and cytotoxicity in murine macrophages were evaluated. SLNs presented size below 200 nm, spherical morphology, negative charge surface, high encapsulation efficiency, above 80%, and excellent stability. Moreover, Fourier transform infrared spectroscopy (FTIR) and differential scanning calorimetry (DSC) analyses were performed to evaluate the chemical structure and possible interactions between DETC and SLNs. SLNs provided a protection for DETC, decreasing its cytotoxic effects in macrophages, which led to an improvement in the selectivity against the parasites, which almost doubled from free DETC (11.4) to DETC incorporated in SLNs (18.2). These results demonstrated that SLNs had a direct effect on L. amazonensis promastigotes without affect the viability of macrophage cell, can be a promising alternative therapy for the cutaneous treatment of L. amazonensis. | pt_BR |
| Affilliation | Federal University of Santa Catarina. Department of Chemical Engineering and Food Engineering. Salvador, BA, Brazil. | pt_BR |
| Affilliation | Federal University of Santa Catarina. Department of Chemical Engineering and Food Engineering. Salvador, BA, Brazil. | pt_BR |
| Affilliation | Federal University of Santa Catarina. Department of Chemical Engineering and Food Engineering. Salvador, BA, Brazil. | pt_BR |
| Affilliation | Federal University of Santa Catarina. Department of Chemical Engineering and Food Engineering. Salvador, BA, Brazil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
| Affilliation | State University of Londrina. Center of Biological Sciences. Laboratory of Experimental Protozoology. Department of Pathological Sciences. Londrina, PR, Brazil. | pt_BR |
| Affilliation | State University of Londrina. Center of Biological Sciences. Laboratory of Experimental Protozoology. Department of Pathological Sciences. Londrina, PR, Brazil. | pt_BR |
| Affilliation | Federal University of Santa Catarina. Department of Chemical Engineering and Food Engineering. Salvador, BA, Brazil. | pt_BR |
| Affilliation | Federal University of Santa Catarina. Department of Chemical Engineering and Food Engineering. Salvador, BA, Brazil. | pt_BR |
| Subject | Solid lipid nanoparticles | pt_BR |
| Subject | Diethyldithiocarbamate | pt_BR |
| Subject | Hydrophilic drugs | pt_BR |
| Subject | Beeswax | pt_BR |
| Subject | L. amazonensis | pt_BR |
