| Author | Trindade, Gabriela das Graças Gomes | |
| Author | Thrivikraman, Greeshma | |
| Author | Menezes, Paula P. | |
| Author | França, Cristiane M. | |
| Author | Lima, Bruno dos Santos | |
| Author | Carvalho, Yasmim M. B. G. | |
| Author | Souza, Eloísa P. B. S. S. | |
| Author | Duarte, Marcelo C. | |
| Author | Shanmugam, Saravanan | |
| Author | Quintans Júnior, Lucindo José | |
| Author | Bezerra, Daniel Pereira | |
| Author | Bertassoni, Luiz E. | |
| Author | Araújo, Adriano Antunes de Souza | |
| Access date | 2019-02-18T14:28:03Z | |
| Available date | 2019-02-18T14:28:03Z | |
| Document date | 2019 | |
| Citation | TRINDADE, G. G. G. et al. Carvacrol/β-cyclodextrin inclusion complex inhibits cell proliferation and migration of prostate cancer cells. Food and Chemical Toxicology, v. 125, p. 198–209, 2019. | pt_BR |
| ISSN | 0278-6915 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/31702 | |
| Sponsorship | CAPES (grant numbers 88881.134368/2016-01 and 88881.134337/
2016–01), CNPq and FAPITEC/SE | pt_BR |
| Language | eng | pt_BR |
| Publisher | Elsevier | pt_BR |
| Rights | restricted access | pt_BR |
| Subject in Portuguese | Carvacrol | pt_BR |
| Subject in Portuguese | Complexo de inclusão | pt_BR |
| Subject in Portuguese | β-ciclodextrina | pt_BR |
| Subject in Portuguese | Viabilidade celular | pt_BR |
| Title | Carvacrol/β-cyclodextrin inclusion complex inhibits cell proliferation and migration of prostate cancer cells | pt_BR |
| Type | Article | |
| DOI | 10.1016/j.fct.2019.01.003 | |
| Abstract | Carvacrol, a phenolic monoterpene derived from thyme oil has gained wide interest recently because of its anticancer activities. To improve the solubility of carvacrol, the formation of inclusion complexes with β-cyclodextrin was performed by ultrasound and freeze-drying methods and characterized using thermal analysis, FTIR, XRD, SEM, NMR and HPLC analysis. From these results, carvacrol was successfully complexed within β-cyclodextrin cavity. Moreover, HPLC analysis demonstrated a higher entrapment efficiency for freeze-drying method (81.20 ± 0.52%) in contrast to ultrasound method (34.02 ± 0.67%). Hence, freeze-drying inclusion complex was evaluated for its antiproliferative effect and cytotoxicity against prostate cancer cell line (PC3) in vitro. Further, freeze-drying complex led to a dose-dependent inhibition in tumor cell growth in 2D and 3D cell culture systems. Altogether, the inclusion of carvacrol in β-cyclodextrin led to the formation of stable complexes with potent antiproliferative effects against PC3 cells, in vitro. Such an improved cytotoxic effect can be attributed to the enhanced the aqueous solubility and bioavailability of carvacrol by effective complexation in β-cyclodextrin. | pt_BR |
| Affilliation | Federal University of Sergipe. Department of Pharmacy. São Cristóvão, SE, Brazil. | pt_BR |
| Affilliation | Oregon Health and Science University. School of Dentistry. Department of Restorative Dentistry. Division of Biomaterials and Biomechanics. Portland, OR, United States. | pt_BR |
| Affilliation | Federal University of Sergipe. Department of Pharmacy. São Cristóvão, SE, Brazil. | pt_BR |
| Affilliation | Oregon Health and Science University. School of Dentistry. Department of Restorative Dentistry. Division of Biomaterials and Biomechanics. Portland, OR, United States. | pt_BR |
| Affilliation | Federal University of Sergipe. Department of Pharmacy. São Cristóvão, SE, Brazil. | pt_BR |
| Affilliation | Federal University of Sergipe. Department of Pharmacy. São Cristóvão, SE, Brazil. | pt_BR |
| Affilliation | Federal University of Sergipe. Department of Pharmacy. São Cristóvão, SE, Brazil. | pt_BR |
| Affilliation | Federal University of Sergipe. Department of Pharmacy. São Cristóvão, SE, Brazil. | pt_BR |
| Affilliation | Federal University of Sergipe. Department of Pharmacy. São Cristóvão, SE, Brazil. | pt_BR |
| Affilliation | Federal University of Sergipe. Department of Physiology. São Cristóvão, SE, Brazil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Oregon Health and Science University. School of Dentistry. Department of Restorative Dentistry. Division of Biomaterials and Biomechanics. Portland, OR, United States / Oregon Health and Science University. Department of Biomedical Engineering. Collaborative Life Science Building. Portland, OR, United States / Oregon Health and Science University. Center for Regenerative Medicine. Portland, OR, United States / Knight Cancer Institute. Cancer Early Detection Advanced Research. Portland, OR, United States. | pt_BR |
| Affilliation | Federal University of Sergipe. Department of Pharmacy. São Cristóvão, SE, Brazil. | pt_BR |
| Subject | Carvacrol | pt_BR |
| Subject | Inclusion complex | pt_BR |
| Subject | β-cyclodextrin | pt_BR |
| Subject | Cell viability | pt_BR |
