| Author | Pereira, Daniela de Pita | |
| Author | Alves, Carlos Roberto | |
| Author | Souza, Marcos Barbosa | |
| Author | Brazil, Reginaldo Peçanha | |
| Author | Bertho, Alvaro Luiz | |
| Author | Barbosa, André de Figueiredo | |
| Author | Britto, Constança Carvalho | |
| Access date | 2019-02-28T10:37:49Z | |
| Available date | 2019-02-28T10:37:49Z | |
| Document date | 2005 | |
| Citation | PEREIRA, Daniela de Pita; et al. Identification of naturally infected Lutzomyia intermedia and Lutzomyia migonei with Leishmania (Viannia) braziliensis in Rio de Janeiro (Brazil) revealed by a PCR multiplex non-isotopic hybridisation assay. Transactions of the Royal Society of Tropical Medicine and Hygiene, v.99, p.905—913, 2005. | pt_BR |
| ISSN | 0035-9203 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/31906 | |
| Language | eng | pt_BR |
| Publisher | Elsevier | pt_BR |
| Rights | restricted access | |
| Subject in Portuguese | Leishmania (Viannia) braziliensis | pt_BR |
| Subject in Portuguese | Lutzomyia spp. | pt_BR |
| Subject in Portuguese | Ensaio multiplex de PCR | pt_BR |
| Subject in Portuguese | Análise de manchas de pontos | pt_BR |
| Subject in Portuguese | Hibridização não isotópica | pt_BR |
| Subject in Portuguese | Brasil | pt_BR |
| Title | Identification of naturally infected Lutzomyia intermedia and Lutzomyia migonei with Leishmania (Viannia) braziliensis in Rio de Janeiro (Brazil) revealed by a PCR multiplex non-isotopic hybridisation assay | pt_BR |
| Type | Article | |
| DOI | 10.1016/j.trstmh.2005.06.019 | |
| Abstract | To identify naturally infected Lutzomyia spp. by Leishmania (Viannia) braziliensis, a PCR multiplex non-isotopic hybridisation assay was developed for the analysis of insect samples collected in distinct areas of the municipality of Rio de Janeiro (Brazil), from March to December 2003. Data from experimental infection indicate that the method can detect one individual infected insect out of ten. Wild sand flies were classified and grouped into pools of 10 specimens each, reaching a total of 40 female groups. Positive results were obtained with pools of Lu. intermedia (5/32) and Lu. migonei (3/5) collected in two areas from the district of Jacarepaguá presenting recent cases of human and canine leishmaniasis. Considering eight infected groups (8/40) with at least one positive insect in each, it was possible to infer an infection rate of 2%. This technique permits the synchronous processing of a large number of samples, in order to investigate infection rates in sand fly populations and to identify potential insect vectors. The results presented here represent the first molecular approach used to infer the natural infection index in both Lutzomyia spp. and constitute essential data to the understanding of leishmaniasis ecoepidemiology in endemic areas from Rio de Janeiro. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Escola Nacional de Saúde Pública Sergio Arouca. Departamento de Ciências Biológicas. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Imunologia. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Entomologia. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Subject | Leishmania (Viannia) braziliensis | pt_BR |
| Subject | Lutzomyia spp. | pt_BR |
| Subject | PCR multiplex assay | pt_BR |
| Subject | Dot blot analysis | pt_BR |
| Subject | Non-isotopic hybridisation | pt_BR |
| Subject | Brazil | pt_BR |
| Embargo date | 2022-01-01 | |
