| Author | Graham, Robert I. | |
| Author | Zahner, Viviane | |
| Author | Lucarotti, Christopher J. | |
| Access date | 2019-02-28T13:00:41Z | |
| Available date | 2019-02-28T13:00:41Z | |
| Document date | 2008 | |
| Citation | GRAHAM, Robert I.; ZAHNER, Viviane; LUCAROTTI, Christopher J. An intracellular symbiont and other microbiota associated with field-collected populations of sawflies (Hymenoptera: Symphyta). Can. J. Microbiol., v.54, p.758–768, 2008. | pt_BR |
| ISSN | 0008-4166 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/31916 | |
| Language | eng | pt_BR |
| Publisher | NRC Research Press | pt_BR |
| Rights | restricted access | |
| Subject in Portuguese | Bactéria | pt_BR |
| Subject in Portuguese | Wolbachia. | pt_BR |
| Subject in Portuguese | endosimbionte | pt_BR |
| Subject in Portuguese | Hymenoptera | pt_BR |
| Subject in Portuguese | moscas | pt_BR |
| Title | An intracellular symbiont and other microbiota associated with field-collected populations of sawflies (Hymenoptera: Symphyta) | pt_BR |
| Type | Article | |
| DOI | 10.1139/W08-067 | |
| Abstract | Six species of sawfly (Hymenoptera: Symphyta) from four taxonomic families (Agridae, Diprionidae, Pamphiliidae,
and Tenthredinidae) were collected from locations across Canada and surveyed for their associated microbiota. Total
DNA was extracted from individual insects, and polymerase chain reaction (PCR) was used to amplify the conserved
16S rRNA gene from microbiota. Denaturing gradient gel electrophoresis (DGGE) and restriction fragment length polymorphism
(RFLP) were undertaken to separate bacterial clones associated with the host insect. Sequencing of the PCR–
DGGE and PCR–RFLP products revealed a dominance of a- and g-Proteobacteria, with most sequences showing high
similarity to bacteria previously identified from other insect species and environmental samples. Additionally, a strain of
the bacterial endosymbiont Wolbachia and a Wolbachia bacteriophage were identified from the mountain ash sawfly (Pristiphora
geniculata). | pt_BR |
| Affilliation | University of New Brunswick. Faculty of Forestry and Environmental Management. Population Ecology Group. Fredericton, Canada. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Celular. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | University of New Brunswick. Faculty of Forestry and Environmental Management. Population Ecology Group. Fredericton, Canada / Canadian Forest Service. Atlantic Forestry Centre. Natural Resources Canada. Fredericton, Canada. | pt_BR |
| xmlui.metadata.dc.description.abstractfr | Six espe`ces de mouches a` scie (Hyme´nopte`res : Symphytes) appartenant a` quatre familles taxonomiques
(Agridae, Diprionidae, Pamphiliidae et Tenthredinidae) ont e´te´ recueillies dans diffe´rentes localite´s a` travers le Canada et
ont e´te´ examine´es quant a` la pre´sence de microbiotes associe´s. L’ADN total a e´te´ extrait des insectes individuellement et
une re´action en chaine par polyme´rase (PCR) a e´te´ utilise´e pour amplifier la re´gion conserve´e du ge`ne de l’ARNr 16S des
microbiotes. Des e´lectrophore`ses sur gel en gradient de´naturant (DGGE) et des analyses de polymorphisme de longueur
des fragments de restriction (RFLP) ont e´te´ re´alise´es pour se´parer les clones bacte´riens associe´s aux insectes hoˆtes. Le se´-
quenc¸age des produits obtenus en PCR–DGGE et en PCR–RFLP a re´ve´le´ une dominance des prote´obacte´ries a et g, la
plupart des se´quences montrant un haut degre´ de similarite´ avec les bacte´ries identifie´es pre´ce´demment chez d’autres insectes
et chez d’autres e´chantillons environnementaux. De plus, une souche de l’endosymbiote bacte´rien Wolbachia et un
bacte´riophage de Wolbachia ont e´te´ identifie´s du Tenthre`de du sorbier (Pristiphora geniculata). | pt_BR |
| Subject | Bacteria | pt_BR |
| Subject | endosymbiont | pt_BR |
| Subject | Hymenoptera | pt_BR |
| Subject | sawflies | pt_BR |
| Subject | Wolbachia | pt_BR |
| Subject in French | Bactérie | pt_BR |
| Subject in French | Wolbachia. | pt_BR |
| Subject in French | Endosymbiote | pt_BR |
| Subject in French | Hyménoptère | pt_BR |
| Subject in French | Mouches a` scie | pt_BR |
| e-ISSN | 1480-3275 | |
| Embargo date | 2030-01-01 | |
